Genome sequencing of the mpox virus 2022 outbreak with amplicon-based Oxford Nanopore MinION sequencing

被引：2
|
作者：
Brinkmann, Annika [1 ,2 ]
Pape, Katharina [1 ,2 ]
Uddin, Steven [1 ,2 ]
Woelk, Niklas [1 ,2 ]
Foerster, Sophie [1 ,2 ]
Jessen, Heiko [3 ]
Michel, Janine [1 ,2 ]
Kohl, Claudia [1 ,2 ]
Schaade, Lars [1 ,2 ]
Nitsche, Andreas [1 ,2 ]
机构：
[1] Robert Koch Inst, Ctr Biol Threats & Special Pathogens, WHO Reference Lab SARS CoV, Highly Pathogen Viruses, See str 10, D-13353 Berlin, Germany
[2] Robert Koch Inst, WHO Collaborating Ctr Emerging Infect & Biol Threa, German Consultant Lab Poxviruses, See str 10, D-13353 Berlin, Germany
[3] Praxis Jessen & Colleagues, Motz str 19, D-10777 Berlin, Germany
来源：
JOURNAL OF VIROLOGICAL METHODS | 2024年 / 325卷
关键词：
Orthopox viruses; Mpox virus; MinION; Nanopore sequencing; TO-HUMAN TRANSMISSION; MONKEYPOX;
D O I：
10.1016/j.jviromet.2024.114888
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We present an amplicon-based assay for MinION Nanopore sequencing of mpox virus (MPXV) genomes from clinical specimens, obtaining high-quality results with an average genome coverage of 99% for Ct values of up to 25, and a genome coverage of 97.1% for Ct values from 25 to 30 which are challenging to sequence. This assay is easy to implement in PCR-based workflows and provides accurate genomic data within a short time.
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收藏
页数：4
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