Protocol for next-generation sequencing of the LSD virus genome using an amplicon-based approach

被引：0
|
作者：
Bhoyar, Rahul C. [1 ]
Jolly, Bani [1 ,2 ]
Vignesh, Harie [1 ]
Bhatt, Lenin [2 ,3 ]
Senthivel, Vigneshwar [1 ,2 ]
Israni, Ravi [2 ]
Scaria, Vinod [1 ,2 ]
Sivasubbu, Sridhar [1 ,2 ]
机构：
[1] Inst Genom & Integrat Biol IGIB, CSIR, Mathura Rd, Delhi 110025, India
[2] Acad Sci & Innovat Res AcSIR, Ghaziabad 201002, Uttar Pradesh, India
[3] State Dis Diagnost Ctr, Dept Anim Husb, Jaipur 302001, Rajasthan, India
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 03期
关键词：
D O I：
10.1016/j.xpro.2024.103020
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Lumpy skin disease (LSD) is a viral disease predominantly affecting cattle caused by a poxvirus belonging to the capripoxvirus genus. Here, we present a protocol for next-generation sequencing of the LSD virus genome using an ampliconbased approach. We describe steps for DNA extraction, viral DNA enrichment, amplicon pooling and purification, and library preparation and pooling. We then detail procedures for sequencing and computational analysis. This protocol can be adapted to any Illumina sequencing platform as an accelerated and scalable system. For complete details on the use and execution of this protocol, please refer to Bhatt et al.1,2 1,2
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页数：13
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