Rapid quantitative detection system for measles virus-neutralizing antibodies using HiBiT-tagged virus-like particles

被引：0

作者：

Okura, Takashi ^{[1
]}

Miyakawa, Kei ^{[2
]}

Tahara, Maino ^{[1
]}

Someya, Kenji ^{[1
]}

Seki, Fumio ^{[1
]}

Nishi, Mayuko ^{[1
]}

Otsuki, Noriyuki ^{[1
]}

Ryo, Akihide ^{[1
,3
]}

机构：

[1] Natl Inst Infect Dis, Dept Virol 3, Tokyo, Japan

[2] Natl Inst Infect Dis, Ctr Influenza & Resp Virus Res, Tokyo, Japan

[3] Natl Inst Infect Dis, Dept Virol 3, Tokyo 2080011, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2024年 / 68卷 / 04期

基金：

日本学术振兴会;

关键词：

fusion; hemagglutinin; HiBiT; LgBiT; measles virus; virus-like particles; virus neutralization test; GROWTH;

D O I：

10.1111/1348-0421.13122

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Immunological testing to detect neutralizing antibodies (NAbs) is important in measles (MV) infection control. Currently, the plaque reduction neutralization test is the only credible method for measuring actual virus NAbs; however, its feasibility is hampered by drawbacks, such as long turnaround times, low throughput, and the need for laboratory biosafety equipment. To solve these problems, we developed a simple and rapid MV-NAb detection system using lentivirus-based virus-like particles incorporated with the NanoLuc fragment peptide HiBiT comprising the MV fusion protein and hemagglutinin on their exterior surface. Overall, this simple, safe, and rapid method could be used to detect MV NAbs.

引用

页码：160 / 164

页数：5

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