A Metal Organic Framework-Based Light Scattering ELISA for the Detection of Staphylococcal Enterotoxin B

被引:1
|
作者
Mao, Kai [1 ]
Tian, Lili [1 ]
Luo, Yujie [1 ]
Li, Qian [2 ]
Chen, Xi [1 ]
Zhan, Lei [2 ]
Li, Yuanfang [1 ]
Huang, Chengzhi [2 ]
Zhen, Shujun [1 ]
机构
[1] Southwest Univ, Minist Educ, Coll Chem & Chem Engn, Key Lab Luminescence Anal & Mol Sensing, Chongqing 400715, Peoples R China
[2] Southwest Univ, Coll Pharmaceut Sci, Chongqing Sci & Technol Bur, Key Lab Luminescent & Real Time Analyt Chem, Chongqing 400715, Peoples R China
关键词
ELISA; light scattering; metal organic framework; ZIF-8; staphylococcal enterotoxin B; PARTICLES; DNA; BIOSENSOR; ZIF-8;
D O I
10.3390/chemosensors11080453
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Enzyme-linked immunosorbent assay (ELISA) is one of the most commonly used method for the detection of staphylococcal enterotoxin B (SEB), the main protein toxin causing staphylococcal food poisoning. However, the traditional ELISA reaction needs to be stopped by sulfuric acid to obtain stable colorimetric signal, and it is easily influenced by a colored sample. In order to address this problem, a new ELISA method using zeolite imidazolate skeleton-8 metal-organic framework (ZIF-8 MOF) as a light scattering (LS) reporter for SEB detection was developed in this work. ZIF-8 MOF has the characteristics of high porosity, large specific surface area, clear pore structure, and adjustable size, which is one of the most representative MOFs constructed from Zn2+ and 2-methylimidazole (2-mIM). The 2-mIM ligand of ZIF-8 exhibited antioxidant activity and can strongly react with H2O2, which could destroy the structure of ZIF-8, resulting in the obvious decrease in LS intensity. We combined this specific reaction with the sandwich immune reaction to construct the LS ELISA method for the successful detection of SEB. This method is more reliable than commercial tests kits for the detection of colored samples, and it is simple, sensitive, and selective, and has great potential in the detection of other toxins by simply changing the corresponding recognition units.
引用
收藏
页数:13
相关论文
共 50 条
  • [41] Metal-Organic Framework-Based Surface-Enhanced Raman Scattering Sensing Platform for Trace Malondialdehyde Detection in Tears
    Li, Jinming
    Yu, Haozhe
    Zhao, Jianming
    Qiao, Xuezhi
    Chen, Xiangyu
    Lu, Zhaoxiang
    Li, Qiaoyu
    Lin, Haimiao
    Wu, Wenyu
    Zeng, Weizhen
    Yang, Zhou
    Feng, Yun
    NANO LETTERS, 2024, 24 (25) : 7792 - 7799
  • [42] APPLICATION OF SANDWICH-ELISA WITH LABELED ANTIBODY FOR DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN-A, ENTEROTOXIN-B, ENTEROTOXIN-C, AND ENTEROTOXIN-D IN FOODS
    WINDEMANN, H
    BAUMGARTNER, E
    ZENTRALBLATT FUR BAKTERIOLOGIE MIKROBIOLOGIE UND HYGIENE SERIE B-UMWELTHYGIENE KRANKENHAUSHYGIENE ARBEITSHYGIENE PRAVENTIVE MEDIZIN, 1985, 181 (3-5): : 345 - 363
  • [43] Metal organic framework-based antibacterial agents and their underlying mechanisms
    Han, Donglin
    Liu, Xiangmei
    Wu, Shuilin
    CHEMICAL SOCIETY REVIEWS, 2022, 51 (16) : 7138 - 7169
  • [44] Microenvironment Modulation in Metal-Organic Framework-Based Catalysis
    Jiao, Long
    Wang, Jingxue
    Jiang, Hai-Long
    ACCOUNTS OF MATERIALS RESEARCH, 2021, 2 (05): : 327 - 339
  • [45] Metal-organic framework-based nanomaterials for biomedical applications
    Zhang, Shu
    Pei, Xibo
    Gao, Huile
    Chen, Song
    Wang, Jian
    CHINESE CHEMICAL LETTERS, 2020, 31 (05) : 1060 - 1070
  • [46] Metal–Organic Framework-Based Sensors for Environmental Contaminant Sensing
    Xian Fang
    Boyang Zong
    Shun Mao
    Nano-Micro Letters, 2018, 10 (04) : 92 - 110
  • [47] Biodegradable Metal-Organic Framework-Based Microrobots (MOFBOTs)
    Terzopoulou, Anastasia
    Wang, Xiaopu
    Chen, Xiang-Zhong
    Palacios-Corella, Mario
    Pujante, Carlos
    Herrero-Martin, Javier
    Qin, Xiao-Hua
    Sort, Jordi
    deMello, Andrei J.
    Nelson, Bradley J.
    Puigmarti-Luis, Josep
    Pane, Salvador
    ADVANCED HEALTHCARE MATERIALS, 2020, 9 (20)
  • [48] Metal–Organic Framework-Based Sulfur-Loaded Materials
    Meng Du
    Qing Li
    Guangxun Zhang
    Feifei Wang
    Huan Pang
    Energy & Environmental Materials, 2022, 5 (01) : 215 - 230
  • [49] Metal-Organic Framework-based Nanozymes and Their Applications in Bioanalysis
    Ma, Xiao
    Zhao, Dan
    Wu, Pei-Cheng
    Lin, Ji-Hong
    Wang, Fang
    Xu, Yan-Jie
    He, Long-Long
    Liu, Xin-Yu
    Sun, Jian
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2023, 51 (06) : 922 - 933
  • [50] Electronically conductive metal-organic framework-based materials
    Kung, Chung-Wei
    Han, Po-Chun
    Chuang, Cheng-Hsun
    Wu, Kevin C. -W.
    APL MATERIALS, 2019, 7 (11)