A Metal Organic Framework-Based Light Scattering ELISA for the Detection of Staphylococcal Enterotoxin B

被引:1
|
作者
Mao, Kai [1 ]
Tian, Lili [1 ]
Luo, Yujie [1 ]
Li, Qian [2 ]
Chen, Xi [1 ]
Zhan, Lei [2 ]
Li, Yuanfang [1 ]
Huang, Chengzhi [2 ]
Zhen, Shujun [1 ]
机构
[1] Southwest Univ, Minist Educ, Coll Chem & Chem Engn, Key Lab Luminescence Anal & Mol Sensing, Chongqing 400715, Peoples R China
[2] Southwest Univ, Coll Pharmaceut Sci, Chongqing Sci & Technol Bur, Key Lab Luminescent & Real Time Analyt Chem, Chongqing 400715, Peoples R China
关键词
ELISA; light scattering; metal organic framework; ZIF-8; staphylococcal enterotoxin B; PARTICLES; DNA; BIOSENSOR; ZIF-8;
D O I
10.3390/chemosensors11080453
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Enzyme-linked immunosorbent assay (ELISA) is one of the most commonly used method for the detection of staphylococcal enterotoxin B (SEB), the main protein toxin causing staphylococcal food poisoning. However, the traditional ELISA reaction needs to be stopped by sulfuric acid to obtain stable colorimetric signal, and it is easily influenced by a colored sample. In order to address this problem, a new ELISA method using zeolite imidazolate skeleton-8 metal-organic framework (ZIF-8 MOF) as a light scattering (LS) reporter for SEB detection was developed in this work. ZIF-8 MOF has the characteristics of high porosity, large specific surface area, clear pore structure, and adjustable size, which is one of the most representative MOFs constructed from Zn2+ and 2-methylimidazole (2-mIM). The 2-mIM ligand of ZIF-8 exhibited antioxidant activity and can strongly react with H2O2, which could destroy the structure of ZIF-8, resulting in the obvious decrease in LS intensity. We combined this specific reaction with the sandwich immune reaction to construct the LS ELISA method for the successful detection of SEB. This method is more reliable than commercial tests kits for the detection of colored samples, and it is simple, sensitive, and selective, and has great potential in the detection of other toxins by simply changing the corresponding recognition units.
引用
下载
收藏
页数:13
相关论文
共 50 条
  • [21] Metal-Organic Framework-Based Enzyme Biocomposites
    Liang, Weibin
    Wied, Peter
    Carraro, Francesco
    Sumby, Christopher J.
    Nidetzky, Bernd
    Tsung, Chia-Kuang
    Falcaro, Paolo
    Doonan, Christian J.
    CHEMICAL REVIEWS, 2021, 121 (03) : 1077 - 1129
  • [22] Advances in metal-organic framework-based membranes
    Cheng, Youdong
    Datta, Shuvo Jit
    Zhou, Sheng
    Jia, Jiangtao
    Shekhah, Osama
    Eddaoudi, Mohamed
    CHEMICAL SOCIETY REVIEWS, 2022, 51 (19) : 8300 - 8350
  • [23] Metal–organic framework-based affinity materials in proteomics
    Adeela Saeed
    Dilshad Hussain
    Shafaq Saleem
    Sehrish Mehdi
    Rabia Javeed
    Fahmida Jabeen
    Muhammad Najam-ul-Haq
    Analytical and Bioanalytical Chemistry, 2019, 411 : 1745 - 1759
  • [24] Applications of metal-organic framework-based bioelectrodes
    Aggarwal, Vidushi
    Solanki, Shipra
    Malhotra, Bansi D.
    CHEMICAL SCIENCE, 2022, 13 (30) : 8727 - 8743
  • [25] Metal-Organic Framework-Based Hybrid Frameworks
    Shan, Yuying
    Chen, Liyu
    Pang, Huan
    Xu, Qiang
    SMALL STRUCTURES, 2021, 2 (02):
  • [26] Metal-Organic Framework-Based Nanomaterials for Electrocatalysis
    Mahmood, Asif
    Guo, Wenhan
    Tabassum, Hassina
    Zou, Ruqiang
    ADVANCED ENERGY MATERIALS, 2016, 6 (17)
  • [27] Metal-organic framework-based heterojunctions for photocatalysis
    Tai, Ran
    Wu, Runjie
    Zhang, Mingzhu
    Yuan, Jie
    Tressel, John
    Tang, Yao
    Wang, Qiang
    Chen, Shaowei
    CURRENT OPINION IN CHEMICAL ENGINEERING, 2024, 45
  • [28] Metal-Organic Framework-Based Cascade Catalysis-Enabled Fluorescent ELISA with Higher Enzymatic Stability for Zearalenone Detection in Maize
    Chen, Chaochao
    Luo, Liang
    Shen, Jianzhong
    Wang, Zhanhui
    Pan, Yantong
    Journal of Agricultural and Food Chemistry, 2024,
  • [29] A simple and ultrasensitive metal-organic framework-based aptasensor for fluorescence detection of ethanolamine
    Khoshbin, Zahra
    Zamanian, Javad
    Davoodian, Negin
    Danesh, Noor Mohammad
    Ramezani, Mohammad
    Alibolandi, Mona
    Abnous, Khalil
    Taghdisi, Seyed Mohammad
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2022, 267
  • [30] Metal-Organic Framework-Based Fiber Optic Sensor for Chromium(VI) Detection
    Menon, Swetha
    Usha, Sruthi Prasood
    Manoharan, Hariharan
    Kishore, Pabbisetti Vayu Nandana
    Sai, V. V. R.
    ACS SENSORS, 2023, 8 (02) : 684 - 693