Identification of autophagy-associated circRNAs in sepsis-induced cardiomyopathy of mice

被引:1
|
作者
Zheng, Ming-zhi [1 ,2 ]
Lou, Jun-sheng [3 ]
Fan, Yun-peng [3 ]
Fu, Chun-yan [4 ,5 ]
Mao, Xing-jia [6 ,7 ]
Li, Xiang [2 ]
Zhong, Kai [2 ]
Lu, Lin-huizi [7 ,8 ]
Wang, Lin-lin [6 ,7 ]
Chen, Ying-ying [4 ,5 ]
Zheng, Liang-rong [1 ]
机构
[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Cardiol & Atrial Fibrillat Ctr, Hangzhou, Peoples R China
[2] Hangzhou Med Coll, Dept Pharmacol, Hangzhou 310053, Peoples R China
[3] Zhejiang Univ, Affiliated Hosp 1, Dept Orthoped Surg, Sch Med, Hangzhou 310003, Peoples R China
[4] Zhejiang Univ, Affiliated Hosp 2, Dept Basic Med Sci, Sch Med, Hangzhou, Peoples R China
[5] Zhejiang Univ, Affiliated Hosp 2, Dept Obstet, Sch Med, Hangzhou, Peoples R China
[6] Zhejiang Univ Sch Med, Dept Basic Med Sci, & Dept Orthopaed Sir Run Run Shaw Hosp, Hangzhou 310016, Peoples R China
[7] Zhejiang Univ Sch Med, Dept Orthopaed Sir Run Run Shaw Hosp, Hangzhou 310016, Peoples R China
[8] Hangzhou Med Coll, Dept Clin Med, Hangzhou, Peoples R China
来源
SCIENTIFIC REPORTS | 2023年 / 13卷 / 01期
关键词
CELLULAR AUTOPHAGY; RAT-LIVER; LYSOSOMES; INJURY;
D O I
10.1038/s41598-023-38998-7
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Circular RNAs (circRNAs) play a role in sepsis-related autophagy. However, the role of circRNAs in autophagy after sepsis-induced cardiomyopathy (SICM) is unknown, so we explored the circRNA expression profiles associated with autophagy in an acute sepsis mouse model. At a dose of 10 mg/kg, mice were intraperitoneally administered with lipopolysaccharides. The myocardial tissue was harvested after 6 h for microarray analysis, qRT-PCR, and western blotting. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes and Gene Set Enrichment Analysis were evaluated, and a competing endogenous RNA network was constructed, to evaluate the role of circRNAs related to autophagy in SICM. In total, 1,735 differently expressed circRNAs were identified in the LPS-treated group, including 990 upregulated and 745 downregulated circRNAs. The expression level of the autophagy-specific protein p62 decreased, while the ratio of LC3 II to LC3 I increased. Additionally, 309 mRNAs and 187 circRNAs were correlated with autophagy in myocardial tissue after SICM. Of these, 179 circRNAs were predicted to function as "miRNA sponges". Some distinctive circRNAs and mRNAs found by ceRNA analysis might be involved in autophagy in SICM. These findings provide insights into circRNAs and identified new research targets that may be used to further explore the pathogenesis of SICM.
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页数:12
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