Establishment of cancer cell line originating from a patient with high-grade serous ovarian carcinoma

被引：1
|
作者：
Linh, Nguyen Thuy [1 ,2 ,3 ]
Hang, Ngo Thu [1 ]
Cuong, Bui Khac [1 ,4 ]
Linh, Dang Thuy [1 ]
Linh, Nham Thi Phuong [4 ]
Nguyen-Van, Do [5 ]
Dzung, Tran Ngoc [2 ]
Mao, Can Van [1 ]
Chung, Dang Thanh [2 ]
Chinh, Le Tri [6 ]
Hung, Nguyen Phu [7 ]
Tong, Hoang Van [1 ,8 ]
Toan, Nguyen Linh [1 ]
机构：
[1] Vietnam Mil Med Univ, Dept Pathophysiol, Hanoi, Vietnam
[2] Vietnam Mil Med Univ, Mil Hosp 103, Dept Pathol, Hanoi, Vietnam
[3] Hanoi Med Univ, Dept Pathol, Hanoi, Vietnam
[4] Vietnam Mil Med Univ, Lab Anim Res Ctr, Hanoi, Vietnam
[5] Hanoi Med Univ, Dept Pathophysiol & Immunol, Hanoi, Vietnam
[6] Vietnam Natl Canc Hosp, Dept Gynaecol Surg, Hanoi, Vietnam
[7] Thai Nguyen Univ Sci, Fac Biotechnol, Thai Nguyen, Vietnam
[8] Vietnam Mil Med Univ, Inst Biomed & Pharm, Hanoi, Vietnam
来源：
FUTURE SCIENCE OA | 2023年 / 9卷 / 08期
关键词：
CD46; cell line; high-grade serous carcinoma; immunohistochemistry; ovarian cancer;
D O I：
10.2144/fsoa-2023-0025
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Aim: Ovarian cancer is a serious malignancy with high prevalence and mortality. Methods: We isolated and characterized an ovarian high-grade serous cancer cell line (M4) from a tumor of a Vietnamese patient with ovarian carcinoma. Results: The M4 cancer cell line showed good proliferation and stability in culture. Morphologically, the M4 cells showed similar characteristics to tumor cells such as a polyhedral shape, large irregular nuclei, high nuclear/cytoplasmic ratio, high nuclear density and expressing cancer markers like CA125, p53 and Ki67 markers. Conclusion: We have successfully isolated and characterized the M4 cell line from a Vietnamese patient with ovarian carcinoma.
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页数：13
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