Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope

被引：1
|
作者：
Peng, Ziyuan [1 ]
Jiao, Yuling [2 ,3 ]
Wang, Ying [1 ]
机构：
[1] Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
[2] Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China
[3] Peking Univ, Acad Adv Interdisciplinary Studies, Peking Tsinghua Ctr Life Sci, Ctr Quantitat Biol, Beijing 100871, Peoples R China
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 02期
关键词：
Developmental biology; Microscopy; Model Organisms; Plant sciences;
D O I：
10.1016/j.xpro.2023.102217
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Live imaging through confocal laser scanning microscopy enables the recording, analysis, and comparison of the dynamics of shapes and gene expression pat-terns of plant shoot apical meristems (SAMs) or primordia. Here, we provide a protocol to describe the preparation process of imaging Arabidopsis SAMs and primordia using a confocal microscope. We describe steps for dissection, visualization of meristems using dyes and fluorescent proteins, and gain 3D morphology of meristems. We then detail analysis of shoot meristems using time-lapse imaging. For complete details on the use and execution of this protocol, please refer to Peng et al. (2022).1
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页数：11
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