Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope

Live imaging through confocal laser scanning microscopy enables the recording, analysis, and comparison of the dynamics of shapes and gene expression pat-terns of plant shoot apical meristems (SAMs) or primordia. Here, we provide a protocol to describe the preparation process of imaging Arabidopsis SAMs and primordia using a confocal microscope. We describe steps for dissection, visualization of meristems using dyes and fluorescent proteins, and gain 3D morphology of meristems. We then detail analysis of shoot meristems using time-lapse imaging. For complete details on the use and execution of this protocol, please refer to Peng et al. (2022).1