Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes

被引：3

作者：

Ichikawa, Takehiko ^{[1
]}

Alam, Mohammad Shahidul ^{[2
]}

Penedo, Marcos ^{[3
]}

Matsumoto, Kyosuke ^{[4
]}

Fujita, Sou ^{[4
]}

Miyazawa, Keisuke ^{[1
,4
]}

Furusho, Hirotoshi ^{[1
]}

Miyata, Kazuki ^{[1
,2
,4
]}

Nakamura, Chikashi ^{[5
]}

Fukuma, Takeshi ^{[1
,2
,4
]}

机构：

[1] Kanazawa Univ, Nano Life Sci Inst WPI NanoLSI, Kakuma Machi, Kanazawa 9201192, Japan

[2] Kanazawa Univ, Grad Sch Frontier Sci Initiat, Div Nano Life Sci, Kakuma Machi, Kanazawa 9201192, Japan

[3] Ecole Polytech Fed Lausanne, Inst Bioengn, Lab Bio & Nanoinstrumentat, CH-1015 Lausanne, Switzerland

[4] Kanazawa Univ, Fac Frontier Engn, Kakuma Machi, Kanazawa 9201192, Japan

[5] Natl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst CMB, AIST INDIA Diverse Assets & Applicat Int Lab DAILA, Tsukuba, Ibaraki 3058565, Japan

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 03期

关键词：

CELLS;

D O I：

10.1016/j.xpro.2023.102468

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Atomic force microscopy (AFM) is capable of nanoscale imaging but has so far only been used on cell surfaces when applied to a living cell. Here, we describe a step-by-step protocol for nanoendoscopy-AFM, which enables the imaging of nanoscale structures inside living cells. The protocol consists of cell staining, fabrication of the nanoneedle probes, observation inside living cells using 2D and 3D nanoendoscopy-AFM, and visualization of the 3D data.For complete details on the use and execution of this protocol, please refer to Penedo et al. (2021)1 and Penedo et al. (2021).2

引用

页数：20

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