Multi-organ single-cell transcriptomics of immune cells uncovered organ-specific gene expression and functions

被引:0
|
作者
Tsagiopoulou, Maria [1 ]
Rashmi, Sonal [1 ]
Aguilar-Fernandez, Sergio [1 ]
Nieto, Juan [1 ]
Gut, Ivo G. [1 ,2 ]
机构
[1] Ctr Nacl Anal Genom CNAG, Barcelona, Spain
[2] Univ Barcelona UB, Barcelona, Spain
基金
欧盟地平线“2020”;
关键词
ATLAS; REGULATOR;
D O I
10.1038/s41597-024-03152-z
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Despite the wealth of publicly available single-cell datasets, our understanding of distinct resident immune cells and their unique features in diverse human organs remains limited. To address this, we compiled a meta-analysis dataset of 114,275 CD45+ immune cells sourced from 14 organs in healthy donors. While the transcriptome of immune cells remains relatively consistent across organs, our analysis has unveiled organ-specific gene expression differences (GTPX3 in kidney, DNTT and ACVR2B in thymus). These alterations are linked to different transcriptional factor activities and pathways including metabolism. TNF-alpha signaling through the NFkB pathway was found in several organs and immune compartments. The presence of distinct expression profiles for NFkB family genes and their target genes, including cytokines, underscores their pivotal role in cell positioning. Taken together, immune cells serve a dual role: safeguarding the organs and dynamically adjusting to the intricacies of the host organ environment, thereby actively contributing to its functionality and overall homeostasis.
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页数:13
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