Evaluation of Omnigene-Sputum for Preservation of Sputum Samples for Diagnosis of Mycobacterium tuberculosis

被引:1
|
作者
Mambuque, Edson [1 ]
Saavedra, Belen [1 ,2 ]
Molina-Moya, Barbara [3 ,4 ]
Nguenha, Dinis [1 ]
Garcia-Garcia, Esther [3 ,4 ]
Blanco, Silvia [1 ]
Gomes, Neide [1 ]
Ehrlich, Joanna [2 ]
Bulo, Helder [1 ]
Munguambe, Shilzia [1 ]
Chiconela, Helio [1 ,5 ]
Acacio, Sozinho [1 ]
Dominguez, Jose [3 ,4 ]
Garcia-Basteiro, Alberto L. [1 ,2 ,6 ]
机构
[1] Ctr Invest Saude Manhica CISM, Maputo 1929, Mozambique
[2] Univ Barcelona, Hosp Clin, ISGlobal, Barcelona 08026, Spain
[3] Univ Autonoma Barcelona, Inst Invest Germans Trias & Pujol, Badalona 08916, Spain
[4] Ctr Invest Biomed Red Enfermedades Resp CIBERES, Badalona 08916, Spain
[5] Natl TB Control Program PNCT, Maputo 1929, Mozambique
[6] Ctr Invest Biomed Red Enfermedades Infecciosas CIB, Barcelona 08026, Spain
关键词
omnigene; sample transportation; tuberculosis; CULTURE; HIV;
D O I
10.3390/tropicalmed8070367
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
In several low-income countries, the transport of sputa could take up to one week to reach the laboratories, resulting in increased contamination rates and a loss of growth. The aim of this study was to evaluate the effect of the OMNIgene-SPUTUM in preserving Mycobacterium tuberculosis on sputum samples simulating three hypothetical scenarios for conservation and/or decontamination: (1) sputum was mixed with OMN and conserved at room temperature for five days and then processed for culture (OMN); (2) sputum cultures followed the routine standing operating procedure at day 0 (STD); and (3) sputum samples were kept at room temperature for five days and mixed with the standard decontamination reagent (SDT5) and then processed for culture. The positivity rate based on smear microscopy was 36.4%, 29.1%, and 27.3% for STD, STD5, and OMN, respectively. The proportion of positive results by liquid culture (MGIT) was 39.1% (43/110) for STD, 26.4% (29/110) for STD5, and 20.0% for OMN (22/110). The overall concordance of liquid culture results was 51.8% (57/110): 37.3% (41/110) for negative results, 11.8% (13/110) for MTBC growth, and 2.7% (3/110) for contaminated results. The OMN arm showed better performance in solid culture than in liquid culture, with a notable reduction in contaminated results.
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页数:10
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