A computational bridge between traction force microscopy and tissue contraction

被引:3
|
作者
Flanary, Shannon M. [1 ]
Jo, Seokwon [2 ]
Ravichandran, Rohit [1 ]
Alejandro, Emilyn U. [2 ]
Barocas, Victor H. [3 ]
机构
[1] Univ Minnesota, Dept Chem Engn & Mat Sci, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Integrat Biol & Physiol, Minneapolis, MN 55455 USA
[3] Univ Minnesota, Dept Biomed Engn, Minneapolis, MN 55455 USA
关键词
VASCULAR SMOOTH-MUSCLE; THORACIC AORTIC-ANEURYSMS; EXTRACELLULAR-MATRIX; STRESS; MODEL;
D O I
10.1063/5.0157507
中图分类号
O59 [应用物理学];
学科分类号
摘要
Arterial wall active mechanics are driven by resident smooth muscle cells, which respond to biological, chemical, and mechanical stimuli and activate their cytoskeletal machinery to generate contractile stresses. The cellular mechanoresponse is sensitive to environmental perturbations, often leading to maladaptation and disease progression. When investigated at the single cell scale, however, these perturbations do not consistently result in phenotypes observed at the tissue scale. Here, a multiscale model is introduced that translates microscale contractility signaling into a macroscale, tissue-level response. The microscale framework incorporates a biochemical signaling network along with characterization of fiber networks that govern the anisotropic mechanics of vascular tissue. By incorporating both biochemical and mechanical components, the model is more flexible and more broadly applicable to physiological and pathological conditions. The model can be applied to both cell and tissue scale systems, allowing for the analysis of in vitro, traction force microscopy and ex vivo, isometric contraction experiments in parallel. When applied to aortic explant rings and isolated smooth muscle cells, the model predicts that active contractility is not a function of stretch at intermediate strain. The model also successfully predicts cell-scale and tissue-scale contractility and matches experimentally observed behaviors, including the hypercontractile phenotype caused by chronic hyperglycemia. The connection of the microscale framework to the macroscale through the multiscale model presents a framework that can translate the wealth of information already collected at the cell scale to tissue scale phenotypes, potentially easing the development of smooth muscle cell-targeting therapeutics.
引用
收藏
页数:15
相关论文
共 50 条
  • [41] 3D Viscoelastic traction force microscopy
    Toyjanova, Jennet
    Hannen, Erin
    Bar-Kochba, Eyal
    Darling, Eric M.
    Henann, David L.
    Franck, Christian
    SOFT MATTER, 2014, 10 (40) : 8095 - 8106
  • [42] Recent advances in biological uses of traction force microscopy
    Youngbin Cho
    Eun Young Park
    Eunmin Ko
    Jin-Sung Park
    Jennifer H. Shin
    International Journal of Precision Engineering and Manufacturing, 2016, 17 : 1401 - 1412
  • [43] Force traction microscopy: An inverse problem with pointwise observations
    Vitale, G.
    Preziosi, L.
    Ambrosi, D.
    JOURNAL OF MATHEMATICAL ANALYSIS AND APPLICATIONS, 2012, 395 (02) : 788 - 801
  • [44] Traction Force Microscopy for Noninvasive Imaging of Cell Forces
    Mulligan, Jeffrey A.
    Bordeleau, Francois
    Reinhart-King, Cynthia A.
    Adie, Steven G.
    BIOMECHANICS IN ONCOLOGY, 2018, 1092 : 319 - 349
  • [45] Traction Force Microscopy to Study B Lymphocyte Activation
    Kumari, Anita
    Pineau, Judith
    Lennon-Dumenil, Ana-Maria
    Balland, Martial
    Pierobon, Paolo
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2020, (161): : 1 - 15
  • [46] Traction force microscopy - Measuring the forces exerted by cells
    Lekka, Malgorzata
    Gnanachandran, Kajangi
    Kubiak, Andrzej
    Zielinski, Tomasz
    Zemla, Joanna
    MICRON, 2021, 150
  • [47] pyTFM: A tool for traction force and monolayer stress microscopy
    Bauer, Andreas
    Prechova, Magdalena
    Fischer, Lena
    Thievessen, Ingo
    Gregor, Martin
    Fabry, Ben
    PLOS COMPUTATIONAL BIOLOGY, 2021, 17 (06)
  • [48] Combined Traction Force-Atomic Force Microscopy Measurements of Neuronal Cells
    Kumarasinghe, Udathari
    Fox, Lucian N.
    Staii, Cristian
    BIOMIMETICS, 2022, 7 (04)
  • [49] Correlation between viscoelastic material properties and contractile prestress of living cells revealed by combined atomic force microscopy (AFM) and traction force microscopy (TEM).
    Schierbaum, N.
    Rheinlaender, J.
    Schaeffer, T. E.
    MOLECULAR BIOLOGY OF THE CELL, 2018, 29 (26)
  • [50] Combined atomic force microscopy (AFM) and traction force microscopy (TFM) reveals a correlation between viscoelastic material properties and contractile prestress of living cells
    Schierbaum, Nicolas
    Rheinlaender, Johannes
    Schaeffer, Tilman E.
    SOFT MATTER, 2019, 15 (08) : 1721 - 1729