A computational bridge between traction force microscopy and tissue contraction

被引:3
|
作者
Flanary, Shannon M. [1 ]
Jo, Seokwon [2 ]
Ravichandran, Rohit [1 ]
Alejandro, Emilyn U. [2 ]
Barocas, Victor H. [3 ]
机构
[1] Univ Minnesota, Dept Chem Engn & Mat Sci, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Integrat Biol & Physiol, Minneapolis, MN 55455 USA
[3] Univ Minnesota, Dept Biomed Engn, Minneapolis, MN 55455 USA
关键词
VASCULAR SMOOTH-MUSCLE; THORACIC AORTIC-ANEURYSMS; EXTRACELLULAR-MATRIX; STRESS; MODEL;
D O I
10.1063/5.0157507
中图分类号
O59 [应用物理学];
学科分类号
摘要
Arterial wall active mechanics are driven by resident smooth muscle cells, which respond to biological, chemical, and mechanical stimuli and activate their cytoskeletal machinery to generate contractile stresses. The cellular mechanoresponse is sensitive to environmental perturbations, often leading to maladaptation and disease progression. When investigated at the single cell scale, however, these perturbations do not consistently result in phenotypes observed at the tissue scale. Here, a multiscale model is introduced that translates microscale contractility signaling into a macroscale, tissue-level response. The microscale framework incorporates a biochemical signaling network along with characterization of fiber networks that govern the anisotropic mechanics of vascular tissue. By incorporating both biochemical and mechanical components, the model is more flexible and more broadly applicable to physiological and pathological conditions. The model can be applied to both cell and tissue scale systems, allowing for the analysis of in vitro, traction force microscopy and ex vivo, isometric contraction experiments in parallel. When applied to aortic explant rings and isolated smooth muscle cells, the model predicts that active contractility is not a function of stretch at intermediate strain. The model also successfully predicts cell-scale and tissue-scale contractility and matches experimentally observed behaviors, including the hypercontractile phenotype caused by chronic hyperglycemia. The connection of the microscale framework to the macroscale through the multiscale model presents a framework that can translate the wealth of information already collected at the cell scale to tissue scale phenotypes, potentially easing the development of smooth muscle cell-targeting therapeutics.
引用
收藏
页数:15
相关论文
共 50 条
  • [21] Field Guide to Traction Force Microscopy
    Denisin, Aleksandra K.
    Kim, Honesty
    Riedel-Kruse, Ingmar H.
    Pruitt, Beth L.
    CELLULAR AND MOLECULAR BIOENGINEERING, 2024, 17 (02) : 87 - 106
  • [22] Tissue traction microscopy to quantify muscle contraction within precision-cut lung slices
    Ram-Mohan, Sumati
    Bai, Yan
    Schaible, Niccole
    Ehrlicher, Allen J.
    Cook, Daniel P.
    Suki, Bela
    Stoltz, David A.
    Solway, Julian
    Ai, Xingbin
    Krishnan, Ramaswamy
    AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2020, 318 (02) : L323 - L330
  • [23] A detailed protocol for cell force measurement by traction force microscopy
    Zhang M.
    Zhang Y.
    Wang P.
    Sun Q.
    Wang X.
    Cao Y.
    Wei Q.
    Smart Materials in Medicine, 2024, 5 (01): : 106 - 113
  • [24] Confocal reference free traction force microscopy
    Bergert, Martin
    Lendenmann, Tobias
    Zundel, Manuel
    Ehret, Alexander E.
    Panozzo, Daniele
    Richner, Patrizia
    Kim, David K.
    Kress, Stephan J. P.
    Norris, David J.
    Sorkine-Hornung, Olga
    Mazza, Edoardo
    Poulikakos, Dimos
    Ferrari, Aldo
    NATURE COMMUNICATIONS, 2016, 7
  • [25] High-Resolution Traction Force Microscopy
    Plotnikov, Sergey V.
    Sabass, Benedikt
    Schwarz, Ulrich S.
    Waterman, Clare M.
    QUANTITATIVE IMAGING IN CELL BIOLOGY, 2014, 123 : 367 - 394
  • [26] Traction force microscopy of engineered cardiac tissues
    Pasqualini, Francesco Silvio
    Agarwal, Ashutosh
    O'Connor, Blakely Bussie
    Liu, Qihan
    Sheehy, Sean P.
    Parker, Kevin Kit
    PLOS ONE, 2018, 13 (03):
  • [27] Traction force microscopy for understanding cellular mechanotransduction
    Hur, Sung Sik
    Jeong, Ji Hoon
    Ban, Myung Jin
    Park, Jae Hong
    Yoon, Jeong Kyo
    Hwang, Yongsung
    BMB REPORTS, 2020, 53 (02) : 74 - 81
  • [28] Confocal reference free traction force microscopy
    Martin Bergert
    Tobias Lendenmann
    Manuel Zündel
    Alexander E. Ehret
    Daniele Panozzo
    Patrizia Richner
    David K. Kim
    Stephan J. P. Kress
    David J. Norris
    Olga Sorkine-Hornung
    Edoardo Mazza
    Dimos Poulikakos
    Aldo Ferrari
    Nature Communications, 7
  • [29] Cell traction force microscopy in cardiac morphogenesis
    Boczonadi, Veronika
    Chen, Victoria
    Henderson, Deborah
    Chaudhry, Bill
    MECHANISMS OF DEVELOPMENT, 2009, 126 : S70 - S71
  • [30] Cellogram: On-the-Fly Traction Force Microscopy
    Lendenmann, Tobias
    Schneider, Teseo
    Dumas, Jeremie
    Tarini, Marco
    Giampietro, Costanza
    Bajpai, Apratim
    Chen, Weiqiang
    Gerber, Julia
    Poulikakos, Dimos
    Ferrari, Aldo
    Panozzo, Daniele
    NANO LETTERS, 2019, 19 (10) : 6742 - 6750