Application of adaptive pressure-driven microfluidic chip in thyroid function measurement

The improvement in accuracy of in vitro diagnosis has always been the focus of early screening of thyroid dysfunction.We constructed a microfluidic chip based on a polystyrene polymer substrate.Total triiodothyronine(TT3),total thyroxine(TT4),free triiodothyronine(FT3),free thyroxine(FT4),and thyrotropin(TSH) in human whole blood samples were analysed by fluorescence immunoassay to evaluate thyroid function.The results indicate that the microfluidic chip shows a good linear relationship in the detection of TT3,TT4,FT3,FT4,and TS H standards,and the correlation coefficient(r) is not less than0.9900.In addition,the chip also has strong anti-interference(RSD%≤5%) and good repeatability(CV≤8%),and its inter-batch differences are small(CV ≤15%).The results of practical application in clinical thyroid function mea surement indicated its high accuracy(r≥0.9900).It provides a new method for the determination of thyroid function and lays a foundation for subsequent clinical application.