Immunocytochemical identification of actin in mitochdria of Physarum polycephalum

被引：0
|
作者：
ZENG Xianlu
Shenzhen University
Department of Biology
Fundamental Medical College
机构：
来源：
Progress in Natural Science | 2003年 / 01期
基金：
中国国家自然科学基金;
关键词：
Physarum polycephalum; mitochondria; actin;
D O I：
暂无
中图分类号：
O656 [有机分析];
学科分类号：
070302 ; 081704 ;
摘要：
Abstract Mitochondria isolated from the plasmodia of Physarum polycephalum Schw. are reacted with rabbit anti-actin anti-body, and detected with FITC-conjugated sheep anti-rabbit IgG antibody. The results of indirect immunofluorescence show that actin ex-ists in the mitochondria. Western blot analysis confirms the existence of actin in the protein preparation of the mitochondria. The indirectimmunoelectron microscopic observation using the same antibodies verifies further that actin is the constituents of mitochondria, and it isdispersively distributed in the mitochondria of P. polycephalum.
引用
收藏
页码：48 / 51
页数：4
相关论文
共 50 条
  • [31] PHYSARUM-POLYCEPHALUM HISTONES
    CORBETT, S
    MILLER, S
    ROBINSON, VJ
    MATTHEWS, HR
    BRADBURY, EM
    BIOCHEMICAL SOCIETY TRANSACTIONS, 1977, 5 (04) : 943 - 946
  • [32] CONTROL OF SPORULATION IN PHYSARUM POLYCEPHALUM
    DANIEL, JW
    RUSCH, HP
    FEDERATION PROCEEDINGS, 1958, 17 (01) : 434 - 434
  • [33] MITOCHONDRIAL DNA IN PHYSARUM POLYCEPHALUM
    KESSLER, D
    JOURNAL OF CELL BIOLOGY, 1969, 43 (2P2): : A68 - &
  • [34] Identification of three genes expressed primarily during development in Physarum polycephalum
    J. Bailey
    L. J. Cook
    R. Kilmer-Barber
    E. Swanson
    L. Solnica-Krezel
    K. Lohman
    W. F. Dove
    J. Dee
    R. W. Anderson
    Archives of Microbiology, 1999, 172 : 364 - 376
  • [35] IDENTIFICATION OF SARCOPLASMIC RETICULUM-LIKE SYSTEM IN PHYSARUM-POLYCEPHALUM
    ZUBRZYCKAGAARN, E
    KORCZAK, B
    OSINSKA, HE
    FEBS LETTERS, 1979, 107 (02): : 335 - 339
  • [36] Microplasmodium Dynamics of Physarum Polycephalum
    Bernitt, E.
    Oettmeier, C.
    Doebereiner, H. -G.
    6TH WORLD CONGRESS OF BIOMECHANICS (WCB 2010), PTS 1-3, 2010, 31 : 1133 - 1136
  • [37] CHANGES IN POLYPHOSPHATE IN PHYSARUM POLYCEPHALUM
    GOODMAN, EM
    SAUER, HW
    RUSCH, HP
    JOURNAL OF CELL BIOLOGY, 1968, 39 (2P2): : A53 - &
  • [38] Manipulating substances with Physarum polycephalum
    Adamatzky, Andrew
    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2010, 30 (08): : 1211 - 1220
  • [39] Identification of transglutaminase substrates in Physarum polycephalum macroplasmodia upon physical damage
    Wada, Fumitaka
    Hasegawa, Hiroki
    Nakamura, Akio
    Shibata, Hideki
    Maki, Masatoshi
    Hitomi, Kiyotaka
    CELL STRUCTURE AND FUNCTION, 2005, 30 : 103 - 103
  • [40] IDENTIFICATION OF A RAS GENE IN THE SLIME-MOLD PHYSARUM-POLYCEPHALUM
    KOZLOWSKI, P
    FRONK, J
    TOCZKO, K
    BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1173 (03) : 357 - 359
12345