Immunocytochemical identification of actin in mitochdria of Physarum polycephalum

被引：0
|
作者：
ZENG Xianlu
Shenzhen University
Department of Biology
Fundamental Medical College
机构：
来源：
Progress in Natural Science | 2003年 / 01期
基金：
中国国家自然科学基金;
关键词：
Physarum polycephalum; mitochondria; actin;
D O I：
暂无
中图分类号：
O656 [有机分析];
学科分类号：
070302 ; 081704 ;
摘要：
Abstract Mitochondria isolated from the plasmodia of Physarum polycephalum Schw. are reacted with rabbit anti-actin anti-body, and detected with FITC-conjugated sheep anti-rabbit IgG antibody. The results of indirect immunofluorescence show that actin ex-ists in the mitochondria. Western blot analysis confirms the existence of actin in the protein preparation of the mitochondria. The indirectimmunoelectron microscopic observation using the same antibodies verifies further that actin is the constituents of mitochondria, and it isdispersively distributed in the mitochondria of P. polycephalum.
引用
收藏
页码：48 / 51
页数：4
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