Expression of Transforming Growth Factor-β in Cultured Normal Human Lens Epithelia Cells

被引：0
|
作者：
黄渝侃
魏厚仁
机构：
[1] Wuhan 430022
[2] Tongji Medical College
[3] Huazhong University of Science and Technology
[4] Department of Ophthalmology
[5] China
[6] Union Hospital
来源：
华中科技大学学报(医学英德文版) | 2004年 / 03期
关键词：
lens; cell culture; transforming growth factor; reverse transcriptase-polymerase chain reaction; immunohistochemistry;
D O I：
暂无
中图分类号：
R329 [人体组织学];
学科分类号：
100101 ;
摘要：
Summary: In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor β (TGF-β), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-β mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-β immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-β, and LEC could be affected by TGF-β through autocrine action.
引用
收藏
页码：289 / 291
页数：3
相关论文
共 50 条
  • [41] Cultured rat glomerular epithelial cells show gene expression and production of transforming growth factor-β:: expression is enhanced by thrombin
    Tsunoda, S
    Yamabe, H
    Osawa, H
    Kaizuka, M
    Shirato, K
    Okumura, K
    NEPHROLOGY DIALYSIS TRANSPLANTATION, 2001, 16 (09) : 1776 - 1782
  • [42] Regulation of basic fibroblast growth factor expression by transforming growth factor beta in cultured human prostate stromal cells
    Story, MT
    Hopp, KA
    Meier, DA
    PROSTATE, 1996, 28 (04): : 219 - 226
  • [43] Expression and regulation of transforming growth factor beta 1 in cultured normal and neoplastic rat pituitary cells
    Qian, X
    Jin, L
    Lloyd, RV
    ENDOCRINE PATHOLOGY, 1996, 7 (01) : 77 - 90
  • [44] Transforming growth factor-β1 induces α-smooth muscle actin expression and fibronectin synthesis in cultured human retinal pigment epithelial cells
    Stocks, SZ
    Taylor, SM
    Shiels, IA
    CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 2001, 29 (01): : 33 - 37
  • [45] Expression of transforming growth factor-β1 and transforming growth factor-β receptors in hepatocellular carcinoma and dysplastic nodules
    Paik, SY
    Park, YN
    Kim, H
    Park, C
    MODERN PATHOLOGY, 2003, 16 (01) : 86 - 96
  • [46] Modulation of endogenous smad expression in normal skin fibroblasts by transforming growth factor-β
    Mori, Y
    Chen, SJ
    Varga, J
    EXPERIMENTAL CELL RESEARCH, 2000, 258 (02) : 374 - 383
  • [47] Dynamic expression patterns of transforming growth factor-β2 and transforming growth factor-β receptors in experimental glomerulonephritis
    Andrea Hartner
    Karl F. Hilgers
    Markus Bitzer
    Roland Veelken
    Harald O. Schöcklmann
    Journal of Molecular Medicine, 2003, 81 : 32 - 42
  • [48] Gene and protein expression changes in human trabecular meshwork cells treated with transforming growth factor-β
    Zhao, XJ
    Ramsey, KE
    Stephan, DA
    Russell, P
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2004, 45 (11) : 4023 - 4034
  • [49] Lovastatin inhibits transforming growth factor-β1 expression in diabetic rat glomeruli and cultured rat mesangial cells
    Kim, SI
    Han, DC
    Lee, HB
    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2000, 11 (01): : 80 - 87
  • [50] Transforming growth factor-β2 gene cloning and protein expression in human trabecular meshwork cells
    Cao Yang
    Wei Houren
    Da Banghong
    Li Zhongyu
    Current Medical Science, 2003, 23 (1) : 85 - 87
12345