Expression of Transforming Growth Factor-β in Cultured Normal Human Lens Epithelia Cells

被引：0
|
作者：
黄渝侃
魏厚仁
机构：
[1] Wuhan 430022
[2] Tongji Medical College
[3] Huazhong University of Science and Technology
[4] Department of Ophthalmology
[5] China
[6] Union Hospital
来源：
华中科技大学学报(医学英德文版) | 2004年 / 03期
关键词：
lens; cell culture; transforming growth factor; reverse transcriptase-polymerase chain reaction; immunohistochemistry;
D O I：
暂无
中图分类号：
R329 [人体组织学];
学科分类号：
100101 ;
摘要：
Summary: In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor β (TGF-β), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-β mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-β immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-β, and LEC could be affected by TGF-β through autocrine action.
引用
收藏
页码：289 / 291
页数：3
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