Strategies for production of active eukaryotic proteins in bacterial expression system

被引：8
|
作者：
Orawan Khow [1 ]
Sunutcha Suntrarachun [1 ]
机构：
[1] Queen Saovabha Memorial Institute,Thai Red Cross Society
来源：
Asian Pacific Journal of Tropical Biomedicine | 2012年 / 02期
关键词：
Recombinant protein; Expression; Escherichia coli;
D O I：
暂无
中图分类号：
Q78 [基因工程（遗传工程）];
学科分类号：
071007 ; 0836 ; 090102 ;
摘要：
Bacteria have long been the favorite expression system for recombinant protein production.However,the flaw of the system is that insoluble and inactive proteins are co-produced due to codon bias,protein folding,phosphorylation,glycosylation,mRNA stability and promoter strength.Factors are cited and the methods to convert to soluble and active proteins are described,for example a tiglit control of Escherichia coli milieu,refolding from inclusion body and through fusion technology.
引用
收藏
页码：159 / 162
页数：4
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