Strategies for production of active eukaryotic proteins in bacterial expression system

被引：8

作者：

Orawan Khow ^{[1
]}

Sunutcha Suntrarachun ^{[1
]}

机构：

[1] Queen Saovabha Memorial Institute,Thai Red Cross Society

来源：

Asian Pacific Journal of Tropical Biomedicine | 2012年 / 02期

关键词：

Recombinant protein; Expression; Escherichia coli;

D O I：

暂无

中图分类号：

Q78 [基因工程（遗传工程）];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

Bacteria have long been the favorite expression system for recombinant protein production.However,the flaw of the system is that insoluble and inactive proteins are co-produced due to codon bias,protein folding,phosphorylation,glycosylation,mRNA stability and promoter strength.Factors are cited and the methods to convert to soluble and active proteins are described,for example a tiglit control of Escherichia coli milieu,refolding from inclusion body and through fusion technology.

引用

页码：159 / 162

页数：4

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