Immunoassay Detection of SARS-CoV-2 Using Monoclonal Antibody Binding to Viral Nucleocapsid Protein

被引：0
|
作者：
Hnasko, Robert M. [1 ]
Lin, Alice V. [1 ]
Mcgarvey, Jeffery A. [2 ]
Jackson, Eric S. [2 ]
机构：
[1] ARS, USDA, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA
[2] ARS, USDA, Foodborne Toxin Detect & Prevent Res Unit, Albany, CA USA
来源：
MICROBIAL BIOTECHNOLOGY | 2025年 / 18卷 / 02期
关键词：
coronavirus; diagnostic; ELISA; immunoassay; lateral flow immunoassay; monoclonal antibodies; nucleocapsid protein; SARS-CoV-2; LENGTH INFECTIOUS CDNA; REVERSE GENETICS; COVID-19; ORIGINS; ASSAY;
D O I：
10.1111/1751-7915.70117
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Immunoassays represent sensitive, easy-to-use, and cost-effective tests useful for the detection of the SARS-CoV-2 virus. In this manuscript, we report on the binding specificity of a pair of novel monoclonal antibodies (MAbs) generated against the SARS-CoV-2 nucleocapsid protein (NP) and their development into sensitive sandwich enzyme-linked immunosorbent assays (sELISA) and a lateral flow immunoassay (LFIA). Binding of these MAbs to hCoVs is limited to variants of SARS-CoV-2 and SARS-CoV NP. Chemiluminescent and absorbance spectroscopy sELISAs report a limit of detection (LOD) for the SARS-CoV-2 B.1.1.529 NP variant at 15 pg/mL, and the LFIA using a red-dyed 200 nm particle at 10 ng/mL. The sELISA exhibits broad SARS-CoV-2 viral variant detection with assay LOD for SARS-CoV-2 B.1.1.529 virus at 1.4 x 105 genome copies per mL (p <= 0.001). The availability of these MAbs should facilitate continued investment in the commercial development of immunoassays to increase global SARS-CoV-2 detection technologies.
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