Biochemical characterization of SARS-CoV-2 nucleocapsid protein

被引：320

作者：

Zeng, Weihong ^{[1
,2
]}

Liu, Guangfeng ^{[3
]}

Ma, Huan ^{[2
]}

Zhao, Dan ^{[2
]}

Yang, Yunru ^{[2
]}

Liu, Muziying ^{[2
]}

Mohammed, Ahmed ^{[2
]}

Zhao, Changcheng ^{[4
]}

Yang, Yun ^{[4
]}

Xie, Jiajia ^{[5
]}

Ding, Chengchao ^{[4
]}

Ma, Xiaoling ^{[6
]}

Weng, Jianping ^{[7
]}

Gao, Yong ^{[4
]}

He, Hongliang ^{[4
]}

Jin, Tengchuan ^{[1
,2
,8
]}

机构：

[1] Univ Sci & Technol China, Affiliated Hosp 1, Dept Obstet & Gynecol, Div Mol Med,Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China

[2] Univ Sci & Technol China, Div Life Sci & Med, Hefei Natl Lab Phys Sci Microscale, Lab Struct Immunol,CAS Key Lab Innate Immun & Chr, Hefei 230027, Anhui, Peoples R China

[3] Chinese Acad Sci, Natl Ctr Prot Sci Shanghai, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China

[4] Univ Sci & Technol China, Affiliated Hosp 1, Dept Infect Dis, Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China

[5] Univ Sci & Technol China, Affiliated Hosp 1, Dept Dermatol, Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China

[6] Univ Sci & Technol China, Affiliated Hosp 1, Dept Lab Med, Div Life Sci & Med, Hefei 230001, Anhui, Peoples R China

[7] Univ Sci & Technol China, Inst Publ Hlth, Hefei 230026, Anhui, Peoples R China

[8] Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai 200031, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 527卷 / 03期

基金：

中国国家自然科学基金;

关键词：

COVID-19; SARS-CoV-2; Nucleocapsid protein; Structure and function; SAXS; Antigenicity; RESPIRATORY-SYNDROME SARS; SYNDROME CORONAVIRUS; DOMAIN; ANTIBODIES;

D O I：

10.1016/j.bbrc.2020.04.136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleocapsid (N) protein is an important antigen for coronavirus, which participate in RNA package and virus particle release. In this study, we expressed the N protein of SARS-CoV-2 and characterized its biochemical properties. Static light scattering, size exclusive chromatography, and small-angle X-ray scattering (SAXS) showed that the purified N protein is largely a dimer in solution. CD spectra showed that it has a high percentage of disordered region at room temperature while it was best structured at 55 degrees C, suggesting its structural dynamics. Fluorescence polarization assay showed it has non-specific nucleic acid binding capability, which raised a concern in using it as a diagnostic marker. Immunoblot assays confirmed the presence of IgA, IgM and IgG antibodies against N antigen in COVID-19 infection patients' sera, proving the importance of this antigen in host immunity and diagnostics. (C) 2020 Published by Elsevier Inc.

引用

页码：618 / 623

页数：6

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