Peptide-sugar ligation catalyzed by transpeptidase sortase: A facile approach to neoglycoconjugate synthesis

被引：0

作者：

Samantaray, Sharmishtha ^{[1
]}

Marathe, Uttara ^{[1
]}

Dasgupta, Sayani ^{[1
]}

Nandicoori, Vinay K. ^{[1
]}

Roy, Rajendra P. ^{[1
]}

机构：

[1] National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India

来源：

Journal of the American Chemical Society | 2008年 / 130卷 / 07期

关键词：

Glycoconjugate synthesis involving sugar and polypeptide remains a formidable challenge. Here we report a novel enzymatic method involving an unprecedented sortase-catalyzed transamidation reaction for site-specific engineering of sugars into native proteins. We show that sugars appended with a 6-aminohexose moiety can be efficiently ligated to peptides and proteins encoded with a LPXTG sortase recognition sequence. This robust reaction provides an elegant and simple approach for generating neoglycoproteins with an amide-linked sugar moiety at the carboxy terminus. Copyright © 2008 American Chemical Society;

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摘要：

Journal article (JA)

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页码：2132 / 2133