On-site visual detection of Nipah virus combining a reverse transcription recombinase-aided amplification with a lateral-flow dipstick assay

被引：0

作者：

Kaikai Jin ^{[1
]}

Junjie Zhao ^{[1
,2
]}

Huanxin Chen ^{[1
]}

Zimo Zhang ^{[1
]}

Zengguo Cao ^{[3
]}

Zanheng Huang ^{[1
]}

Hao Li ^{[1
]}

Yongsai Liu ^{[1
]}

Lisi Ai ^{[1
]}

Yufei Liu ^{[1
]}

Changqi Fan ^{[4
]}

Yuanyuan Li ^{[1
]}

Pei Huang ^{[1
]}

Hualei Wang ^{[1
]}

Haili Zhang ^{[1
]}

机构：

[1] State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory for Zoonosis Research of the Ministry of Education/Institute of Zoonosis, College of Veterinary Medicine, Jilin University

[2] National/WOAH Reference Laboratory for Classical Swine Fever, China Institute of Veterinary Drug Control

[3] Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences

[4] Zhili College, Tsinghua

来源：

Journal of Integrative Agriculture | 2025年 / 24卷 / 02期

关键词：

D O I：

暂无

中图分类号：

S852.65 [家畜病毒学];

学科分类号：

摘要：

<正>Highlights The RT-RAA-VF assay developed for the NiV P gene can perform rapid detection of NiV within 20 min at 42°C with high specificity.This assay is capable of attaining sensitivity to a single copy of NiV RNA transcripts.This assay effectively avoids false positives caused by aerosol contamination with a sealed disposable nucleic acid visualization test paper device.

引用

页码：790 / 794

页数：5

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