On-site visual detection of Nipah virus combining a reverse transcription recombinase-aided amplification with a lateral-flow dipstick assay

被引：0

作者：

Kaikai Jin ^{[1
]}

Junjie Zhao ^{[1
,2
]}

Huanxin Chen ^{[1
]}

Zimo Zhang ^{[1
]}

Zengguo Cao ^{[3
]}

Zanheng Huang ^{[1
]}

Hao Li ^{[1
]}

Yongsai Liu ^{[1
]}

Lisi Ai ^{[1
]}

Yufei Liu ^{[1
]}

Changqi Fan ^{[4
]}

Yuanyuan Li ^{[1
]}

Pei Huang ^{[1
]}

Hualei Wang ^{[1
]}

Haili Zhang ^{[1
]}

机构：

[1] State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory for Zoonosis Research of the Ministry of Education/Institute of Zoonosis, College of Veterinary Medicine, Jilin University

[2] National/WOAH Reference Laboratory for Classical Swine Fever, China Institute of Veterinary Drug Control

[3] Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences

[4] Zhili College, Tsinghua

来源：

Journal of Integrative Agriculture | 2025年 / 24卷 / 02期

关键词：

D O I：

暂无

中图分类号：

S852.65 [家畜病毒学];

学科分类号：

摘要：

<正>Highlights The RT-RAA-VF assay developed for the NiV P gene can perform rapid detection of NiV within 20 min at 42°C with high specificity.This assay is capable of attaining sensitivity to a single copy of NiV RNA transcripts.This assay effectively avoids false positives caused by aerosol contamination with a sealed disposable nucleic acid visualization test paper device.

引用

页码：790 / 794

页数：5

共 50 条

[21] Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus
LI Jiang Shuai
HAO Yan Zhe
HOU Mei Ling
ZHANG Xuan
ZHANG Xiao Guang
CAO Yu Xi
LI Jin Ming
MA Jing
ZHOU Zhi Xiang
Biomedical and Environmental Sciences, 2022, 35 (02) : 133 - 140
[22] Recombinase-aided amplification-lateral flow dipstick assay-a specific and sensitive method for visual detection of avian infectious laryngotracheitis virus
Wang, Wenjing
Wang, Chunguang
Zhang, Zichuang
Zhang, Peng
Zhai, Xianghe
Li, Xinyue
Zhang, Tie
POULTRY SCIENCE, 2021, 100 (03)
[23] Development and application of a recombinase-aided amplification combined with a lateral flow dipstick assay for rapid visual detection of anguillid herpesvirus 1
Chen, Xi
Chen, Hua
Ge, Jun-Qing
JOURNAL OF FISH DISEASES, 2024, 47 (04)
[24] Research Note: Application of reverse-transcription recombinase-aided amplification-lateral flow dipstick method in the detection of infectious bursal disease virus
Wang, Chao
Wang, Chunguang
Zhang, Zongshu
Li, Xinyue
Zhang, Tie
POULTRY SCIENCE, 2023, 102 (01)
[25] Recombinase-aided amplification combined with lateral flow dipstick for the rapid detection of Amphidinium carterae
Meiting Xu
Chunyun Zhang
Fuguo Liu
Yuanyuan Wang
Runqi Li
Guofu Chen
Journal of Applied Phycology, 2022, 34 : 435 - 447
[26] Development of an Internally Controlled Reverse Transcription Recombinase-aided Amplification Assay for the Rapid and Visual Detection of West Nile Virus
Fan Guo Hao
Shen Xin Xin
Li Fan
Li Xin Na
Bai Xue Ding
Zhang Rui Qing
Wang Rui Huan
Lei Wen Wen
Wang Huan Yu
Ma Xue Jun
Wu Gui Zhen
BIOMEDICAL AND ENVIRONMENTAL SCIENCES, 2019, 32 (12) : 926 - +
[27] Recombinase-aided amplification combined with lateral flow dipstick for the rapid detection of Amphidinium carterae
Xu, Meiting
Zhang, Chunyun
Liu, Fuguo
Wang, Yuanyuan
Li, Runqi
Chen, Guofu
JOURNAL OF APPLIED PHYCOLOGY, 2022, 34 (01) : 435 - 447
[28] Development of an Internally Controlled Reverse Transcription Recombinase-aided Amplification Assay for the Rapid and Visual Detection of West Nile Virus
FAN Guo Hao
SHEN Xin Xin
LI Fan
LI Xin Na
BAI Xue Ding
ZHANG Rui Qing
WANG Rui Huan
LEI Wen Wen
WANG Huan Yu
MA Xue Jun
WU Gui Zhen
Biomedical and Environmental Sciences, 2019, 32 (12) : 926 - 929
[29] Development and Application of Recombinase-Aided Amplification Combined with Lateral Flow Dipstick Assay for Rapid Detection of Staphylococcus aureus
Hou L.
Li D.
Deng B.
Zhao Y.
Pan Y.
Feng D.
Sun X.
Shipin Kexue/Food Science, 2022, 43 (04): : 331 - 339
[30] Field applicable detection of hepatitis B virus using internal controlled duplex recombinase-aided amplification assay and lateral flow dipstick assay
Bai, Xueding
Ma, Xuejun
Li, Minghui
Li, Xinna
Fan, Guohao
Zhang, Ruiqing
Wang, Ruihuan
Duan, Qingxia
Shen, Xinxin
Xie, Yao
Rong, Xiuge
JOURNAL OF MEDICAL VIROLOGY, 2020, 92 (12) : 3344 - 3353

← 1 2 3 4 5 →