Determination of Deoxyribonucleic Acid with Rosaniline by a Resonance Light Scattering Method

被引：0
|
作者：
Liu, Chen [1 ]
Chen, Xiao-Ming [1 ]
Xiang, Hai-Yan [1 ]
Li, Songqin [1 ]
Xia, Shu [1 ]
Liu, A.N.-Xi [1 ]
机构：
[1] Inst. of Chem. and Chem. Engineering, Xiangtan University, Xiangtan 411105, China
来源：
Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis | 2001年 / 21卷 / 05期
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摘要：
The resonance light scattering (RLS) spectra of Rosaniline with DMA have been studied. The RLS of Rosaniline is greatly enhanced by DNA in pH range of 10.5-10.8. There is a resonance light scattering peak ofat 485 nm,and the enhanced intensity of RLS at this wavelength is proportional to the concentration of DNA. The linear range of the calibration graph is 0-1.00 mg·L-1 with the correlation coefficient of 0.998 0. The detection limit is 14.2 ng·mL-1.The relatiive standard deviation(n = 10) was within 2.1%. This method is simple, rapid and has been applied to the determination of DNA in mixed samples with satisfactory results.
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页码：699 / 700
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