Determination of Deoxyribonucleic Acid with Rosaniline by a Resonance Light Scattering Method

被引：0

作者：

Liu, Chen ^{[1
]}

Chen, Xiao-Ming ^{[1
]}

Xiang, Hai-Yan ^{[1
]}

Li, Songqin ^{[1
]}

Xia, Shu ^{[1
]}

Liu, A.N.-Xi ^{[1
]}

机构：

[1] Inst. of Chem. and Chem. Engineering, Xiangtan University, Xiangtan 411105, China

来源：

Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis | 2001年 / 21卷 / 05期

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摘要：

The resonance light scattering (RLS) spectra of Rosaniline with DMA have been studied. The RLS of Rosaniline is greatly enhanced by DNA in pH range of 10.5-10.8. There is a resonance light scattering peak ofat 485 nm,and the enhanced intensity of RLS at this wavelength is proportional to the concentration of DNA. The linear range of the calibration graph is 0-1.00 mg·L-1 with the correlation coefficient of 0.998 0. The detection limit is 14.2 ng·mL-1.The relatiive standard deviation(n = 10) was within 2.1%. This method is simple, rapid and has been applied to the determination of DNA in mixed samples with satisfactory results.

引用

页码：699 / 700

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