Development and validation of a new RP-HPLC method for determination of quercetin in green tea

被引：0
|
作者：
机构：
[1] Savic, Ivan M.
[2] Nikolic, Vesna D.
[3] Savic, Ivana M.
[4] Nikolic, Ljubisa B.
[5] Stankovic, Mihajlo Z.
来源：
| 1600年 / Maik Nauka Publishing / Springer SBM卷 / 68期
关键词：
Green tea (Camellia sinensis) contains quercetin as a bioactive compound. Quercetin has anti-inflammatory and anticancer effects. The aim of this paper was to develop and validate an RP-HPLC method for determination of quercetin in green tea simpler and faster than other available methods. RP-HPLC analysis was performed by isocratic elution with a flow rate of 1.0 mL/min. Pure methanol was used as a mobile phase; while the quantification was effected at 370 nm. The separation was performed at 35 C using a C18 column (4.6 × 250 mm; 5 μm). The results showed that the peak area response is linear within the concentration range of 10-70 μg/mL (r = 0.9986). The values of LOD and LOQ were 1.2 and 4 μg/mL; respectively. For the intra-day and intra-instrument reproducibility; RSD were in the range of 0.05-0.84% and 0.89-1.55%; respectively. The results of accuracy for the different concentrations of quercetin (40; 50; and; 60; μg/mL); were; 101.3; 98.4; and 98.2%. The developed and validated method was successfully applied to the determination of quercetin in green tea extract. © 2013 Pleiades Publishing; Ltd;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
相关论文
共 50 条
  • [31] Development and Validation of a RP-HPLC Method for Determination of Citicoline Monosodium in Human Plasma
    Bindaiya, Shailendra K.
    Sahu, Kapendra
    Bhaisare, Mukesh
    Karthikeyan, C.
    Moorthy, N. S. H. N.
    Mehta, Farhad F.
    Trivedi, Piyush
    LATIN AMERICAN JOURNAL OF PHARMACY, 2011, 30 (04): : 794 - 798
  • [32] Development and validation of an RP-HPLC method for simultaneous determination of Ramipril and Amlodipine in tablets
    Shi-Ying Dai
    Shi-Ting Qiu
    Wei Wu
    Chun-Mei Fu
    Journal of Pharmaceutical Analysis, 2013, 3 (06) : 440 - 446
  • [33] Development and validation of an RP-HPLC method for determination of valganciclovir in human serum and tablets
    Dogan-Topal, B.
    Uslu, B.
    Ozkan, S. A.
    CHROMATOGRAPHIA, 2007, 66 (Suppl 1) : S97 - S101
  • [34] Development and Validation of a Simple RP-HPLC Method for Determination of Efavirenz in Polymeric Microparticles
    Lyra, Amanda M.
    Nadal, Jessica M.
    Klein, Traudi
    Farago, Paulo V.
    LATIN AMERICAN JOURNAL OF PHARMACY, 2017, 36 (12): : 2354 - 2362
  • [35] Development and validation of RP-HPLC method for determination of Lornoxicam in rabbit's plasma
    Shah, Syed Nisar Hussain
    Sohail, Kashif
    Javaid, Zeeshan
    Zaman, Muhammad
    Basheer, Ejaz
    PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2019, 32 (01) : 333 - 338
  • [36] METHOD DEVELOPMENT AND VALIDATION OF DONEPEZIL HYDROCHLORIDE BY RP-HPLC
    Dusia, Sharmila
    Ramanamma, L.
    Prasad, D. S. V. S.
    INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2018, 5 (05): : 4228 - 4251
  • [37] Development and validation of the RP-HPLC method for quantification of tavaborole
    Prajapati, Shiv Kumar
    Jain, Ankit
    Bajpai, Meenakshi
    ANALYTICAL METHODS, 2024, 16 (30) : 5280 - 5287
  • [38] Development and Validation of a RP-HPLC Method for Simvastatin Capsules
    Marques-Marinho, Flavia Dias
    Freitas, Bruna Diniz
    da Costa Zanon, Jose Carlos
    Reis, Ilka Afonso
    Lima, Angelica Alves
    Vianna-Soares, Cristina Duarte
    CURRENT PHARMACEUTICAL ANALYSIS, 2013, 9 (01) : 2 - 12
  • [39] METHOD DEVELOPMENT AND VALIDATION OF CAPECITABINE IN TABLETS BY RP-HPLC METHOD
    Rao, M. Prasada
    Leelavathi, K.
    Chandreshta, N.
    Sowjanya, V.
    Sireesha, I.
    Rajani, I.
    Premi, V. V. Naga
    INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2016, 3 (05): : 492 - 507
  • [40] Analytical method development and validation of Artemether by RP-HPLC
    Sumithra, M.
    Surya, S.
    ANNALS OF PHYTOMEDICINE-AN INTERNATIONAL JOURNAL, 2024, 13 (01): : 709 - 714
12345