Development and validation of a new RP-HPLC method for determination of quercetin in green tea

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[1] Savic, Ivan M.

[2] Nikolic, Vesna D.

[3] Savic, Ivana M.

[4] Nikolic, Ljubisa B.

[5] Stankovic, Mihajlo Z.

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| 1600年 / Maik Nauka Publishing / Springer SBM卷 / 68期

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Green tea (Camellia sinensis) contains quercetin as a bioactive compound. Quercetin has anti-inflammatory and anticancer effects. The aim of this paper was to develop and validate an RP-HPLC method for determination of quercetin in green tea simpler and faster than other available methods. RP-HPLC analysis was performed by isocratic elution with a flow rate of 1.0 mL/min. Pure methanol was used as a mobile phase; while the quantification was effected at 370 nm. The separation was performed at 35 C using a C18 column (4.6 × 250 mm; 5 μm). The results showed that the peak area response is linear within the concentration range of 10-70 μg/mL (r = 0.9986). The values of LOD and LOQ were 1.2 and 4 μg/mL; respectively. For the intra-day and intra-instrument reproducibility; RSD were in the range of 0.05-0.84% and 0.89-1.55%; respectively. The results of accuracy for the different concentrations of quercetin (40; 50; and; 60; μg/mL); were; 101.3; 98.4; and 98.2%. The developed and validated method was successfully applied to the determination of quercetin in green tea extract. © 2013 Pleiades Publishing; Ltd;

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