PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays

被引：0

作者：

Stewart, Chloe L. ^{[1
,2
]}

Hook, Andrew L. ^{[1
]}

Zelzer, Mischa ^{[1
]}

Marlow, Maria ^{[1
]}

Piccinini, Anna M. ^{[1
]}

机构：

[1] Univ Nottingham, Sch Pharm, Nottingham, England

[2] Univ Oxford, Inst Dev & Regenerat Med, Oxford, England

来源：

CELL BIOCHEMISTRY AND FUNCTION | 2024年 / 42卷 / 05期

基金：

英国工程与自然科学研究理事会;

关键词：

biomaterial; cytotoxicity; fibroblast; hydrogel; monocyte; viability; THERMOSENSITIVE HYDROGELS; DELIVERY;

D O I：

10.1002/cbf.4097

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We identified that PLGA-PEG-PLGA hydrogels, which have been used in human clinical trials and possess a demonstrable safety profile, induced significant cytotoxicity in conventional in vitro assays. This major contradiction may lead to inconsistent and misleading toxicology due to the limited biological representation of these assays. Cytotoxicity evaluation is a crucial element of screening the biological response to new biomaterials. However, as standard test methods do not recapitulate the in vivo environment, tailored adaptations may be required to reflect the true biological response elicited toward novel biomaterials.

引用

页数：4

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