IMMOBILIZATION OF PENICILLIN-G ACYLASE ON FUNCTIONALIZED MACROPOROUS POLYMER BEADS

被引：33
|
作者：
BAHULEKAR, RV
PRABHUNE, AA
SIVARAMAN, H
PONRATHNAM, S
机构：
[1] NATL CHEM LAB,DIV CHEM ENGN,POONA 411008,MAHARASHTRA,INDIA
[2] NATL CHEM LAB,DIV BIOCHEM SCI,POONA 411008,MAHARASHTRA,INDIA
来源：
POLYMER | 1993年 / 34卷 / 01期
关键词：
BIOCATALYST ENZYME IMMOBILIZATION; MACROPOROUS SUPPORT; POLYETHYLENEIMINE; PENICILLIN ACYLASE;
D O I：
10.1016/0032-3861(93)90300-Y
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Macroporous beaded polymers of varying pore sizes were synthesized coated with polyethyleneimine and derivatized with glutaraldehyde to generate aldehyde pendant groups. Penicillin G acylase (EC 3.5.1.11) was immobilized by covalent binding and the performance of immobilized enzyme was evaluated in batch mode. The expression was estimated as 43%. The shift in pH optima was marginal. The optimum temperature shifted from 40 to 57-degrees-C and K(m) shifted from 31 to 12.8 mumol. Immobilization enhanced the thermal stability of the enzyme.
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页码：163 / 166
页数：4
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