GEL-ENTRAPPED PENICILLIN-G ACYLASE OPTIMIZED BY AN ENZYME THERMISTOR

被引：7
|
作者：
WELWARDOVA, A
GEMEINER, P
MICHALKOVA, E
WELWARD, L
JAKUBOVA, A
机构：
[1] SLOVAK ACAD SCI, INST CHEM, DUBRAVSKA CESTA 9, CS-84238 BRATISLAVA, CZECHOSLOVAKIA
[2] BIOTIKA PHARMACEUT PLANT, CS-97613 SLOVENSKA LUPCA, CZECHOSLOVAKIA
来源：
BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 11期
关键词：
D O I：
10.1007/BF00153750
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Direct activity determination by a flow-through microcalorimetry in the enzyme thermistor system was employed for a fast comparison of (poly)acrylamide gel-entrapped penicillin G acylase preparations. Composition of the pre-polymerization cocktail and both the storage and operational stabilities of optimal gel-entrapped enzyme preparations isolated from the Escherichia coli industrial strain were optimized by this method. The validity of the results was corroborated by spectrophotometric measurements.
引用
收藏
页码：809 / 814
页数：6
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