SOMATOSTATIN BINDING TO DISSOCIATED CELLS FROM RAT CEREBRAL-CORTEX

被引：21

作者：

COLAS, B ^{[1
]}

PRIETO, JC ^{[1
]}

ARILLA, E ^{[1
]}

机构：

[1] UNIV ALCALA DE HENARES, DEPT BIOQUIM & BIOL MOLEC, E-28871 ALCALA DE HENARES, SPAIN

来源：

PEPTIDES | 1990年 / 11卷 / 06期

关键词：

DISSOCIATED BRAIN CELLS; SOMATOSTATIN RECEPTOR; CEREBRAL CORTEX;

D O I：

10.1016/0196-9781(90)90138-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for the study of somatostatin (SS) binding to dissociated cells from rat cerebral cortex. Binding of [I-125][Tyr11]SS to cells obtained by mechanical dissociation of rat cerebral cortex was dependent on time and temperature, saturable, reversible and highly specific. Under conditions of equilibrium, i.e., 60 min at 25-degrees-C, native SS inhibited tracer binding in a dose-dependent manner. The Scatchard analysis of binding data was linear and yielded a dissociation constant of 0.60 +/- 0.08 nM with a maximal binding capacity of 160 +/- 16 fmol/mg protein. The binding of [I-125][Tyr11]SS was specific as shown in experiments on tracer displacement by the native peptide, SS analogues, and unrelated peptides.

引用

页码：1109 / 1112

页数：4

共 50 条

[41] INFLUENCE OF SUCCINYLPURINES ON THE BINDING OF ADENOSINE TO A PARTICULATE FRACTION OF RAT CEREBRAL-CORTEX
VINCENT, MF
VANDENBERGHE, G
[J]. PEDIATRIC RESEARCH, 1988, 24 (01) : 138 - 138
[42] INFLUENCE OF SUCCINYLPURINES ON THE BINDING OF ADENOSINE TO A PARTICULATE FRACTION OF RAT CEREBRAL-CORTEX
VINCENT, MF
VANDENBERGHE, G
[J]. PURINE AND PYRIMIDINE METABOLISM IN MAN VI, PT B: BASIC RESEARCH AND EXPERIMENTAL BIOLOGY, 1989, 253 : 441 - 445
[43] DIURNAL-VARIATION IN RELEASE OF SOMATOSTATIN-LIKE IMMUNOREACTIVITY FROM INCUBATED RAT HYPOTHALAMUS AND CEREBRAL-CORTEX
BERELOWITZ, M
DUDLAK, D
FROHMAN, LA
[J]. ENDOCRINOLOGY, 1982, 110 (06) : 2195 - 2197
[44] DNA TURNOVER IN RAT CEREBRAL-CORTEX
PERRONECAPANO, C
DONOFRIO, G
GIUDITTA, A
[J]. JOURNAL OF NEUROCHEMISTRY, 1982, 38 (01) : 52 - 56
[45] DISTRIBUTION OF DOPAMINE IN RAT CEREBRAL-CORTEX
KEHR, W
LINDQVIST, M
CARLSSON, A
[J]. JOURNAL OF NEURAL TRANSMISSION, 1976, 38 (3-4) : 173 - 180
[46] ACETYLCHOLINESTERASE IN NEURONS OF THE RAT CEREBRAL-CORTEX
TOTH, L
KREUTZBERG, GW
[J]. ACTA ANATOMICA, 1979, 103 (02): : 125 - 129
[47] EFFECTS OF FLUNARIZINE ON RAT CEREBRAL-CORTEX
REID, KH
MARRANNES, R
WAUQUIER, A
[J]. DRUG DEVELOPMENT RESEARCH, 1987, 10 (02) : 107 - 116
[48] STUDIES ON THE VASCULOGENESIS IN RAT CEREBRAL-CORTEX
MATO, M
OOKAWARA, S
NAMIKI, T
[J]. ANATOMICAL RECORD, 1989, 224 (03): : 355 - 364
[49] CHOLINERGIC MECHANISMS IN RAT CEREBRAL-CORTEX
STONE, TW
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1972, 222 (02): : P155 - &
[50] CHOLECYSTOKININ INNERVATION OF RAT CEREBRAL-CORTEX
SEROOGY, KB
FALLON, JH
[J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1985, 448 (JUL) : 636 - 639

← 1 2 3 4 5 →