SOMATOSTATIN BINDING TO DISSOCIATED CELLS FROM RAT CEREBRAL-CORTEX

被引：21

作者：

COLAS, B ^{[1
]}

PRIETO, JC ^{[1
]}

ARILLA, E ^{[1
]}

机构：

[1] UNIV ALCALA DE HENARES, DEPT BIOQUIM & BIOL MOLEC, E-28871 ALCALA DE HENARES, SPAIN

来源：

PEPTIDES | 1990年 / 11卷 / 06期

关键词：

DISSOCIATED BRAIN CELLS; SOMATOSTATIN RECEPTOR; CEREBRAL CORTEX;

D O I：

10.1016/0196-9781(90)90138-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for the study of somatostatin (SS) binding to dissociated cells from rat cerebral cortex. Binding of [I-125][Tyr11]SS to cells obtained by mechanical dissociation of rat cerebral cortex was dependent on time and temperature, saturable, reversible and highly specific. Under conditions of equilibrium, i.e., 60 min at 25-degrees-C, native SS inhibited tracer binding in a dose-dependent manner. The Scatchard analysis of binding data was linear and yielded a dissociation constant of 0.60 +/- 0.08 nM with a maximal binding capacity of 160 +/- 16 fmol/mg protein. The binding of [I-125][Tyr11]SS was specific as shown in experiments on tracer displacement by the native peptide, SS analogues, and unrelated peptides.

引用

页码：1109 / 1112

页数：4

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