In order to establish the method of generating powerful gd T cells for anti-tumor immunotherapy, we investigated the effects of monocyte-derived dendritic cells (mo-DCs) on anti-tumor cytotoxicity of expanded gamma delta T cells. Activation of gamma delta T cells co-cultured for 2-3da ys with immature or mature mo-DCs was evaluated by CD69 expression and anti-tumor cytotoxicity using two assays : the 5-(and 6-) carboxyfluorescein diacetate, succinimidyl ester-based cytotoxicity assay and the calcein-AM-based Terascan assay. gamma delta T cells were used as effector cells and myeloma cell line (RPMI8226) or chronic myelogenous leukemia blastic crisis cell line (C2F8) were used as target cells. CD69 expression on gamma delta T cells was enhanced by co-culture with both immature and mature mo-DCs in a cell-number-dependent fashion. CD69 expression was enhanced after addition of mo-DCs of either autologous or allogeneic origin. Activation of gamma delta T cells with mo-DCs enhanced anti-tumor cytotoxicity of gamma delta T cells against RPMI8226 and C2F8 in an effector-to-target ratio-dependent manner. Activation of gamma delta T cells by mo-DCs was associated with the enhancement of anti-tumor cytotoxicity of gamma delta T cells. Potent gamma delta T cells activated by mo-DCs were considered to be applicable to an efficient gamma delta T cell-mediated immunotherapy for tumors.
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Mil Med Acad, Inst Med Res, Belgrade 11002, SerbiaMil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Rajkovic, Ivan
Dragicevic, Ana
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Mil Med Acad, Inst Med Res, Belgrade 11002, SerbiaMil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Dragicevic, Ana
Vasilijic, Sasa
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Mil Med Acad, Inst Med Res, Belgrade 11002, SerbiaMil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Vasilijic, Sasa
Bozic, Biljana
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Mil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Univ Belgrade, Inst Physiol & Biochem, Belgrade 11001, SerbiaMil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Bozic, Biljana
Dzopalic, Tanja
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Mil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Univ Nis, Med Fac Nis, Nish, SerbiaMil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Dzopalic, Tanja
Tomic, Sergej
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Mil Med Acad, Inst Med Res, Belgrade 11002, SerbiaMil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Tomic, Sergej
Majstorovic, Ivana
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Mil Med Acad, Inst Med Res, Belgrade 11002, SerbiaMil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Majstorovic, Ivana
Vucevic, Dragana
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Mil Med Acad, Inst Med Res, Belgrade 11002, SerbiaMil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Vucevic, Dragana
Djokic, Jelena
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Mil Med Acad, Inst Med Res, Belgrade 11002, SerbiaMil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Djokic, Jelena
Balint, Bela
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Mil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Mil Med Acad, Transfusiol Inst, Belgrade 11002, SerbiaMil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Balint, Bela
Colic, Miodrag
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Mil Med Acad, Inst Med Res, Belgrade 11002, Serbia
Univ Nis, Med Fac Nis, Nish, SerbiaMil Med Acad, Inst Med Res, Belgrade 11002, Serbia
机构:
Johannes Gutenberg Univ Mainz, Clin Res Unit Allergol, Dept Dermatol, Med Ctr, Mainz, GermanyJohannes Gutenberg Univ Mainz, Clin Res Unit Allergol, Dept Dermatol, Med Ctr, Mainz, Germany
Trojandt, S.
Grabbe, S.
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Johannes Gutenberg Univ Mainz, Clin Res Unit Allergol, Dept Dermatol, Med Ctr, Mainz, GermanyJohannes Gutenberg Univ Mainz, Clin Res Unit Allergol, Dept Dermatol, Med Ctr, Mainz, Germany
Grabbe, S.
Reske-Kunz, A. B.
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Johannes Gutenberg Univ Mainz, Clin Res Unit Allergol, Dept Dermatol, Med Ctr, Mainz, GermanyJohannes Gutenberg Univ Mainz, Clin Res Unit Allergol, Dept Dermatol, Med Ctr, Mainz, Germany
Reske-Kunz, A. B.
Bros, M.
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Johannes Gutenberg Univ Mainz, Clin Res Unit Allergol, Dept Dermatol, Med Ctr, Mainz, GermanyJohannes Gutenberg Univ Mainz, Clin Res Unit Allergol, Dept Dermatol, Med Ctr, Mainz, Germany