Enhancement of Anti-tumor Cytotoxicity of Expanded gamma delta T Cells by Stimulation with Monocyte-derived Dendritic Cells

被引:13
|
作者
Saito, Anri [1 ,2 ]
Narita, Miwako [1 ]
Yokoyama, Ayumi [1 ]
Watanabe, Norihiro [1 ,2 ]
Tochiki, Nozomi [1 ]
Satoh, Noriyuki [1 ]
Takizawa, Jun [2 ]
Furukawa, Tatsuo [3 ]
Toba, Ken [2 ]
Fuse, Ichiro [4 ]
Aizawa, Yoshifusa [2 ]
Shinada, Shohji [5 ]
Takahashi, Masuhiro [1 ]
机构
[1] Niigata Univ, Grad Sch Hlth Sci, Lab Hematol & Oncol, Niigata, Japan
[2] Niigata Univ, Grad Sch Med & Dent Sci, Div Hematol, Niigata, Japan
[3] Niigata Univ, Med & Dent Gen Hosp, Div Stem Cell Transplantat, Niigata, Japan
[4] Niigata Univ, Med & Dent Gen Hosp, Biosci Med Res Ctr, Niigata, Japan
[5] Niigata Prefecture Red Cross Blood Ctr, Niigata, Japan
关键词
gamma delta T cells; monocyte-derived dendritic cells; CD69; anti-tumor cytotoxicity; cellular immunotherapy;
D O I
10.3960/jslrt.47.61
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In order to establish the method of generating powerful gd T cells for anti-tumor immunotherapy, we investigated the effects of monocyte-derived dendritic cells (mo-DCs) on anti-tumor cytotoxicity of expanded gamma delta T cells. Activation of gamma delta T cells co-cultured for 2-3da ys with immature or mature mo-DCs was evaluated by CD69 expression and anti-tumor cytotoxicity using two assays : the 5-(and 6-) carboxyfluorescein diacetate, succinimidyl ester-based cytotoxicity assay and the calcein-AM-based Terascan assay. gamma delta T cells were used as effector cells and myeloma cell line (RPMI8226) or chronic myelogenous leukemia blastic crisis cell line (C2F8) were used as target cells. CD69 expression on gamma delta T cells was enhanced by co-culture with both immature and mature mo-DCs in a cell-number-dependent fashion. CD69 expression was enhanced after addition of mo-DCs of either autologous or allogeneic origin. Activation of gamma delta T cells with mo-DCs enhanced anti-tumor cytotoxicity of gamma delta T cells against RPMI8226 and C2F8 in an effector-to-target ratio-dependent manner. Activation of gamma delta T cells by mo-DCs was associated with the enhancement of anti-tumor cytotoxicity of gamma delta T cells. Potent gamma delta T cells activated by mo-DCs were considered to be applicable to an efficient gamma delta T cell-mediated immunotherapy for tumors.
引用
收藏
页码:61 / 72
页数:12
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