ANTISENSE OLIGONUCLEOTIDES - STEREOCONTROLLED SYNTHESIS OF PHOSPHOROTHIOATE OLIGONUCLEOTIDES

被引：14

作者：

SUSKA, A ^{[1
]}

GRAJKOWSKI, A ^{[1
]}

WILK, A ^{[1
]}

UZNANSKI, B ^{[1
]}

BLASZCZYK, J ^{[1
]}

WIECZOREK, M ^{[1
]}

STEC, WJ ^{[1
]}

机构：

[1] TECH UNIV LODZ,INST TECH BIOCHEM,PL-90924 LODZ,POLAND

来源：

PURE AND APPLIED CHEMISTRY | 1993年 / 65卷 / 04期

关键词：

D O I：

10.1351/pac199365040707

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new method of oligo(nucleoside phosphorothioate)s (OligoS) synthesis, based on the oligonucleotide chain extention via DBU-catalyzed reaction of 5'-OH-nucleoside(-tide)with5'-DMT-nucleoside-3'-O-(2-thiono- 1.3.2-oxathiaphospholane)s (2) is elaborated. The process of oxathiaphospholane ring opening is shown to be chemo- and stereoselective. The use of separated diastereoisomers of 2 allows the synthesis of OligoS with a predetermined sense of chirality at each internucleotide phosphorothioate function.

引用

页码：707 / 714

页数：8

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