Efficient synthesis of antisense phosphorothioate oligonucleotides: Evaluation of dichloroacetic acid at higher concentration to reduce cycle time

被引：6

作者：

Cheruvallath, ZS ^{[1
]}

Carty, RL ^{[1
]}

Andrade, M ^{[1
]}

Moore, MN ^{[1
]}

Song, QL ^{[1
]}

Rentel, C ^{[1
]}

Cole, DL ^{[1
]}

Ravikumar, VT ^{[1
]}

机构：

[1] ISIS Pharmaceut, Carlsbad, CA 92008 USA

来源：

ORGANIC PROCESS RESEARCH & DEVELOPMENT | 2003年 / 7卷 / 06期

关键词：

D O I：

10.1021/op030006j

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Efficiency of phosphorothioate oligonucleotide syntheses could be improved by increasing the concentration of dichloroacetic acid in toluene to 10% from 3% (v/v) during the detritylation step. It was also found that dichloroacetic acid is better than dichloropropionic acid when compared at similar concentrations.

引用

页码：917 / 920

页数：4

共 6 条

[1] Efficient synthesis of antisense phosphorothioate oligonucleotides using a universal solid support
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[3] MODIFIED OLIGONUCLEOTIDES .4. SOLID-PHASE SYNTHESIS AND PRELIMINARY EVALUATION OF PHOSPHOROTHIOATE RNA AS POTENTIAL ANTISENSE AGENTS
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RAYNER, B
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[4] Antisense oligonucleotides:: Efficient synthesis of 2′-O-methoxyethyl phosphorothioate oligonucleotides using 4,5-dicyanoimidazole.: Are these oligonucleotides comparable to those synthesized using 1H-tetrazole as coupling activator?
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[5] Synthesis and evaluation of glucuronic acid derivatives as alkylating agents for the reversible masking of internucleoside groups of antisense oligonucleotides
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[6] Efficient Suppression of Tissue Factor Synthesis Using Antisense Oligonucleotides Selected by an Enhanced Strategy for Evaluation of Structural Characteristics
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