ISOLATION AND CHARACTERIZATION OF DNA-SPECIFIC CATALYTIC ANTIBODIES FROM PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS

被引：0
|
作者：
GOLOLOBOV, GV
BOGOMOLOVA, AE
YADAV, RP
ERMOLAEVA, MV
BELOSTOTSKAYA, KM
PROKAEVA, TB
SHUSTER, AM
GABIBOV, AG
机构：
[1] RUSSIAN ACAD MED SCI,INST RHEUMATOL,MOSCOW,RUSSIA
[2] MM SHEMYAKIN INST BIOORGAN CHEM,MOSCOW,RUSSIA
来源：
BIOCHEMISTRY-MOSCOW | 1993年 / 58卷 / 02期
关键词：
CATALYTIC ANTIBODIES; PROTEIN-A; DNA-SPECIFIC ABZYMES;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for isolating DNA-specific catalytic antibodies (natural abzymes) is described Purification procedures include affinity chromatography on protein A Sepharose or DNA-cellulose (the latter using either native DNA or glutaraldehyde-modified DNA as sorbent) and gel-permeation HPLC. Elution conditions were optimized to ensure maximum yields of activity. Biochemical and immunochemical characteristics of the natural DNA-specific abzymes are reported Their interaction with a model substrate and the influence of different effectors on DNA hydrolysis were investigated.
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页码：183 / 187
页数：5
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