CHANGES IN RADIATION SENSITIVITY OF HUMAN OSTEOSARCOMA CELLS AFTER P53 INTRODUCTION

被引：11
|
作者：
MIYAKOSHI, J
YAMAGISHI, N
OHTSU, S
TAKEBE, H
机构：
[1] Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Yoshida‐Konoe‐cho, Kyoto, 606-01, Sakyo-ku
来源：
JAPANESE JOURNAL OF CANCER RESEARCH | 1995年 / 86卷 / 08期
关键词：
P53; INTRODUCTION; EXPRESSION; RADIATION SENSITIVITY; HUMAN OSTEOSARCOMA SAOS-2 CELLS;
D O I：
10.1111/j.1349-7006.1995.tb02457.x
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Human osteosarcoma SAOS-2 cells, which have a deletion in p53 gene, were transfected with plasmid pMSV neop53 containing human p53 cDNA and neomycin-resistance gene. Three clones (SAOS-MC10, SAOS-MC11 and SAOS-MC43) among 60 clones expressed p53 mRNA. No p53 protein was observed in SAOS-MC10, while SAOS-MC11 and SAOS-MC43 produced p53 protein. The molecular weight of p53 protein in SAOS-MC43 was lower than that in SAOS-MC11. SAOS-MC11 and SAOS-MC43 were more sensitive and more resistant, respectively, to ionizing radiation than the parental SAOS-2. We suggest that exogenous p53 protein might be one of the factors determining cellular radiosensitivity.
引用
收藏
页码：711 / 713
页数：3
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