CHANGES IN RADIATION SENSITIVITY OF HUMAN OSTEOSARCOMA CELLS AFTER P53 INTRODUCTION

被引：11
|
作者：
MIYAKOSHI, J
YAMAGISHI, N
OHTSU, S
TAKEBE, H
机构：
[1] Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Yoshida‐Konoe‐cho, Kyoto, 606-01, Sakyo-ku
来源：
JAPANESE JOURNAL OF CANCER RESEARCH | 1995年 / 86卷 / 08期
关键词：
P53; INTRODUCTION; EXPRESSION; RADIATION SENSITIVITY; HUMAN OSTEOSARCOMA SAOS-2 CELLS;
D O I：
10.1111/j.1349-7006.1995.tb02457.x
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Human osteosarcoma SAOS-2 cells, which have a deletion in p53 gene, were transfected with plasmid pMSV neop53 containing human p53 cDNA and neomycin-resistance gene. Three clones (SAOS-MC10, SAOS-MC11 and SAOS-MC43) among 60 clones expressed p53 mRNA. No p53 protein was observed in SAOS-MC10, while SAOS-MC11 and SAOS-MC43 produced p53 protein. The molecular weight of p53 protein in SAOS-MC43 was lower than that in SAOS-MC11. SAOS-MC11 and SAOS-MC43 were more sensitive and more resistant, respectively, to ionizing radiation than the parental SAOS-2. We suggest that exogenous p53 protein might be one of the factors determining cellular radiosensitivity.
引用
收藏
页码：711 / 713
页数：3
相关论文
共 50 条
  • [21] Regulation of P53 stability in p53 mutated human and mouse hepatoma cells
    Hailfinger, Stephan
    Jaworski, Maike
    Marx-Stoelting, Philip
    Wanke, Ines
    Schwarz, Michael
    INTERNATIONAL JOURNAL OF CANCER, 2007, 120 (07) : 1459 - 1464
  • [22] Prognostic value of p53 alterations in human osteosarcoma: a meta analysis
    Yao, Dong
    Cai, Guo-Hong
    Chen, Jing
    Ling, Rui
    Wu, Sheng-Xi
    Li, Yong-Ping
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2014, 7 (10): : 6725 - 6733
  • [23] CKB affects human osteosarcoma progression by regulating the p53 pathway
    Zhou, Fengxin
    Dou, Xinli
    Li, Chenguang
    AMERICAN JOURNAL OF CANCER RESEARCH, 2022, 12 (10): : 4652 - 4665
  • [24] Effect of p53 gene transfection on human hepatocarcinoma cells' sensitivity to irradiation.
    Tao, Zhengchao
    Qian, Liting
    JOURNAL OF CLINICAL ONCOLOGY, 2013, 31 (15)
  • [25] MGMT and p53 status predict temozolomide sensitivity in human malignant glioma cells
    Hermisson, M
    Klumpp, A
    Wick, W
    Wischhusen, J
    Nagel, G
    Roos, W
    Kaina, B
    Weller, M
    NEURO-ONCOLOGY, 2005, 7 (03) : 376 - 376
  • [26] Overexpression of GRIM-19 accelerates radiation-induced osteosarcoma cells apoptosis by p53 stabilization
    Chen, Wanhong
    Liu, Qingbai
    Fu, Bin
    Liu, Kai
    Jiang, Wenchao
    LIFE SCIENCES, 2018, 208 : 232 - 238
  • [27] Wild-type p53 reduces radiation hypermutability in p53-mutated human lymphoblast cells
    Zhang, Qinming
    Liu, Yunfeng
    Zhou, Juntling
    Chen, Weihong
    Zhang, Ying
    Liber, Howard L.
    MUTAGENESIS, 2007, 22 (05) : 329 - 334
  • [28] Inhibition of tumorigenicity in human gastric cancer cells by introduction of exogenous p53 gene
    孙梅
    吕有勇
    Chinese Science Bulletin, 1996, (15) : 1297 - 1302
  • [29] Lack of p53 augments thymoquinone-induced apoptosis and caspase activation in human osteosarcoma cells
    Roepke, M.
    Diestel, A.
    Bajbouj, K.
    Schoenfeld, P.
    Roessner, A.
    Gali-Muhtasib, H.
    Schneider-Stock, R.
    PATHOLOGY RESEARCH AND PRACTICE, 2007, 203 (05) : 340 - 340
  • [30] Extracellular matrix induces doxorubicin-resistance in human osteosarcoma cells by suppression of p53 function
    Harisi, Revekka
    Dudas, Jozsef
    Nagy-Olah, Julia
    Timor, Ferenc
    Szendroi, Miklos
    Jeney, Andras
    CANCER BIOLOGY & THERAPY, 2007, 6 (08) : 1240 - 1246
12345