BIOPSY MEASUREMENT OF DNA DAMAGE AND REPAIR INVIVO - SINGLE-STRAND BREAKS, ALKALI-LABILE BONDS, AND ENDONUCLEASE-SENSITIVE SITES

被引：13

作者：

BRASH, DE ^{[1
]}

HART, RW ^{[1
]}

机构：

[1] OHIO STATE UNIV, DEPT RADIOL, COLUMBUS, OH 43210 USA

来源：

RADIATION RESEARCH | 1982年 / 91卷 / 01期

关键词：

D O I：

10.2307/3575824

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA damage and repair measurement by alkaline sucrose gradient sedimentation was adapted to in vivo biopsy samples by refining a sensitive DNA fluorescence assay and establishing quantitatable sedimentation conditions for high-MW DNA from isolated nuclei. Induction and repair of X-ray-induced single-strand breaks plus alkali-labile bonds in rat liver DNA were measured in whole liver and in successive biopsies taken from single animals; repair half-time was 20-30 min. Incubation of UV-irradiated rat liver nuclei with UV endonuclease before sedimentation enabled detection of pyrimidine dimers.

引用

页码：169 / 180

页数：12

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