BIOPSY MEASUREMENT OF DNA DAMAGE AND REPAIR INVIVO - SINGLE-STRAND BREAKS, ALKALI-LABILE BONDS, AND ENDONUCLEASE-SENSITIVE SITES

被引：13
|
作者：
BRASH, DE [1 ]
HART, RW [1 ]
机构：
[1] OHIO STATE UNIV, DEPT RADIOL, COLUMBUS, OH 43210 USA
来源：
RADIATION RESEARCH | 1982年 / 91卷 / 01期
关键词：
D O I：
10.2307/3575824
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
DNA damage and repair measurement by alkaline sucrose gradient sedimentation was adapted to in vivo biopsy samples by refining a sensitive DNA fluorescence assay and establishing quantitatable sedimentation conditions for high-MW DNA from isolated nuclei. Induction and repair of X-ray-induced single-strand breaks plus alkali-labile bonds in rat liver DNA were measured in whole liver and in successive biopsies taken from single animals; repair half-time was 20-30 min. Incubation of UV-irradiated rat liver nuclei with UV endonuclease before sedimentation enabled detection of pyrimidine dimers.
引用
收藏
页码：169 / 180
页数：12
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