DEVELOPMENT OF A DISPOSABLE AMPEROMETRIC SENSOR FOR REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE BASED ON A CHEMICALLY-MODIFIED SCREEN-PRINTED CARBON ELECTRODE

被引：47

作者：

SPRULES, SD

HART, JP

WRING, SA

PITTSON, R

机构：

[1] UNIV W ENGLAND,FAC APPL SCI,BRISTOL BS16 1QY,AVON,ENGLAND

[2] GLAXO GRP RES LTD,DIV DRUG METAB,WARE SG12 0DP,HERTS,ENGLAND

[3] GWENT ELECTR MAT LTD,PONTYPOOL NP4 0H2,GWENT,WALES

来源：

ANALYST | 1994年 / 119卷 / 02期

关键词：

AMPEROMETRY; SCREEN-PRINTING; MELDOLAS BLUE; NICOTINAMIDE ADENINE DINUCLEOTIDE;

D O I：

10.1039/an9941900253

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The evaluation of novel, disposable, screen-printed electrodes by means of cyclic voltammetry with potassium hexacyanoferrate(II), phenazine methosulfate, and Meldola's Blue is described. Meldola's Blue was incorporated into selected electrodes, and the electrocatalytic oxidation of reduced nicotinamide adenine dinucleotide (NADH) was investigated using cyclic voltammetry and hydrodynamic voltammetry. The modified electrodes were used for quantitative measurements of NADH by means of amperometry in stirred solutions at applied potentials of -0.05 V (versus a saturated calomel electrode). The limit of detection was found to be 2.5 X 10(-6) mol dm(-3); the response was linear up to 2.5 x 10(-4) mol dm(-3).

引用

页码：253 / 257

页数：5

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