ANALYSIS OF THE IRON-SULFUR CLUSTERS WITHIN THE COMPLEX-I (NADH-UBIQUINONE OXIDOREDUCTASE) ISOLATED FROM POTATO-TUBER MITOCHONDRIA

被引：12
|
作者：
LIN, TI
SLED, VD
OHNISHI, T
BRENNICKE, A
GROHMANN, L
机构：
[1] INST GENBIOL FORSCH, D-14195 BERLIN, GERMANY
[2] UNIV ULM, W-7900 ULM, GERMANY
[3] UNIV PENN, DEPT BIOCHEM & BIOPHYS, JOHNSON RES FDN, PHILADELPHIA, PA 19104 USA
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 230卷 / 03期
关键词：
MITOCHONDRIA (PLANT); NADH-UBIQUINONE OXIDOREDUCTASE (COMPLEX I); EPR; IRON-SULFUR CLUSTER; SOLANUM TUBEROSUM;
D O I：
10.1111/j.1432-1033.1995.tb20652.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mitochondrial complex I (NADH:ubiquinone oxidoreductase) isolated from potato (Solanum tuberosum) has been investigated for the presence of iron-sulfur clusters. EPR spectroscopic analysis detected signals arising from clusters N1, N2, N3 and N4. Quantitation of the content of iron and sulfur within the isolated complex I showed the preparation to contain 22.6 mol acid-labile sulfide and 30.4 mol iron/mol complex I. The iron-sulfur cluster composition of the plant complex I appears to be similar to the well-known composition found in Neurospora crassa.
引用
收藏
页码：1032 / 1036
页数：5
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