ANALYSIS OF THE IRON-SULFUR CLUSTERS WITHIN THE COMPLEX-I (NADH-UBIQUINONE OXIDOREDUCTASE) ISOLATED FROM POTATO-TUBER MITOCHONDRIA

被引：12
|
作者：
LIN, TI
SLED, VD
OHNISHI, T
BRENNICKE, A
GROHMANN, L
机构：
[1] INST GENBIOL FORSCH, D-14195 BERLIN, GERMANY
[2] UNIV ULM, W-7900 ULM, GERMANY
[3] UNIV PENN, DEPT BIOCHEM & BIOPHYS, JOHNSON RES FDN, PHILADELPHIA, PA 19104 USA
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 230卷 / 03期
关键词：
MITOCHONDRIA (PLANT); NADH-UBIQUINONE OXIDOREDUCTASE (COMPLEX I); EPR; IRON-SULFUR CLUSTER; SOLANUM TUBEROSUM;
D O I：
10.1111/j.1432-1033.1995.tb20652.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mitochondrial complex I (NADH:ubiquinone oxidoreductase) isolated from potato (Solanum tuberosum) has been investigated for the presence of iron-sulfur clusters. EPR spectroscopic analysis detected signals arising from clusters N1, N2, N3 and N4. Quantitation of the content of iron and sulfur within the isolated complex I showed the preparation to contain 22.6 mol acid-labile sulfide and 30.4 mol iron/mol complex I. The iron-sulfur cluster composition of the plant complex I appears to be similar to the well-known composition found in Neurospora crassa.
引用
收藏
页码：1032 / 1036
页数：5
相关论文
共 50 条
  • [1] DIPHENYLENEIODONIUM INHIBITS REDUCTION OF IRON-SULFUR CLUSTERS IN THE MITOCHONDRIAL NADH-UBIQUINONE OXIDOREDUCTASE (COMPLEX-I)
    MAJANDER, A
    FINEL, M
    WIKSTROM, M
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1994, 269 (33) : 21037 - 21042
  • [2] THERMODYNAMIC ANALYSIS OF FLAVIN IN MITOCHONDRIAL NADH-UBIQUINONE OXIDOREDUCTASE (COMPLEX-I)
    SLED, VD
    RUDNITZKY, NI
    HATEFI, Y
    OHNISHI, T
    BIOCHEMISTRY, 1994, 33 (33) : 10069 - 10075
  • [3] MITOCHONDRIAL NADH-UBIQUINONE OXIDOREDUCTASE (COMPLEX-I) - PROXIMITY OF THE SUBUNITS OF THE FLAVOPROTEIN AND THE IRON SULFUR PROTEIN SUBCOMPLEXES
    YAMAGUCHI, M
    HATEFI, Y
    BIOCHEMISTRY, 1993, 32 (08) : 1935 - 1939
  • [4] IRON-SULFUR N-1 CLUSTERS STUDIED IN NADH-UBIQUINONE OXIDOREDUCTASE AND IN SOLUBLE NADH DEHYDROGENASE
    OHNISHI, T
    BLUM, H
    GALANTE, YM
    HATEFI, Y
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1981, 256 (17) : 9216 - 9220
  • [5] ELECTRON-PARAMAGNETIC-RES STUDIES OF IRON-SULFUR CLUSTERS IN ISOLATED SUBUNITS AND SUBFRACTIONS OF NADH-UBIQUINONE OXIDOREDUCTASE
    OHNISHI, T
    RAGAN, CI
    HATEFI, Y
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1985, 260 (05) : 2782 - 2788
  • [6] MOLECULAR DEFECTS OF NADH-UBIQUINONE OXIDOREDUCTASE (COMPLEX-I) IN MITOCHONDRIAL DISEASES
    MORGANHUGHES, JA
    SCHAPIRA, AHV
    COOPER, JM
    CLARK, JB
    JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1988, 20 (03) : 365 - 382
  • [7] ISOLATION AND CHARACTERIZATION OF SUBCOMPLEXES OF THE MITOCHONDRIAL NADH-UBIQUINONE OXIDOREDUCTASE (COMPLEX-I)
    FINEL, M
    MAJANDER, AS
    TYYNELA, J
    DEJONG, AMP
    ALBRACHT, SPJ
    WIKSTROM, M
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 226 (01): : 237 - 242
  • [8] ATTEMPTS TO DEFINE DISTINCT PARTS OF NADH-UBIQUINONE OXIDOREDUCTASE (COMPLEX-I)
    FRIEDRICH, T
    WEIDNER, U
    NEHLS, U
    FECKE, W
    SCHNEIDER, R
    WEISS, H
    JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1993, 25 (04) : 331 - 337
  • [9] ON THE STOICHIOMETRY OF THE IRON-SULFUR CLUSTERS IN MITOCHONDRIAL NADH - UBIQUINONE OXIDOREDUCTASE
    VANBELZEN, R
    DEJONG, AMP
    ALBRACHT, SPJ
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 209 (03): : 1019 - 1022
  • [10] STUDIES ON THE STRUCTURE OF NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX - TOPOGRAPHY OF THE SUBUNITS OF THE IRON-SULFUR FLAVOPROTEIN COMPONENT
    HAN, AL
    YAGI, T
    HATEFI, Y
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1988, 267 (02) : 490 - 496
12345