CLONING AND NUCLEOTIDE-SEQUENCES OF 2 LIPASE GENES FROM CANDIDA-CYLINDRACEA

被引：75
|
作者：
LONGHI, S [1 ]
FUSETTI, F [1 ]
GRANDORI, R [1 ]
LOTTI, M [1 ]
VANONI, M [1 ]
ALBERGHINA, L [1 ]
机构：
[1] UNIV MILAN, DIPARTIMENTO FISIOL & BIOCHIM GEN, SEZ BIOCHIM COMPARATA, I-20133 MILAN, ITALY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 02期
关键词：
LIPASE GENE; CLONING; GENOMIC SEQUENCE; (YEAST); (C-CYLINDRACEA);
D O I：
10.1016/0167-4781(92)90085-E
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two lipase-encoding genes (LIP1 and LIP2) have been isolated from a SacI genomic library of the yeast Candida cylindracea and their nucleotide sequences have been determined. Comparison with the sequence of a cDNA ruled out the presence of introns in the two genes. Both ORFs encode for mature proteins of 534 residues with putative signal peptides of 15 and 14 amino acids, respectively. When compared with other lipase sequences, the two C. cylindracea lipases showed homology only with the Geotrichum candidum lipase, whereas they shared a significant similarity with several esterases.
引用
收藏
页码：227 / 232
页数：6
相关论文
共 50 条
  • [41] REGIOSELECTIVE ACYLATION OF BILE-ACID DERIVATIVES WITH CANDIDA-CYLINDRACEA LIPASE IN ANHYDROUS BENZENE
    RIVA, S
    BOVARA, R
    OTTOLINA, G
    SECUNDO, F
    CARREA, G
    JOURNAL OF ORGANIC CHEMISTRY, 1989, 54 (13): : 3161 - 3164
  • [42] ENZYME-CATALYZED CONDENSATION POLYMERIZATION OF 11-HYDROXYUNDECANOIC ACID WITH LIPASE FROM CANDIDA-CYLINDRACEA
    OHAGAN, D
    ZAIDI, NA
    POLYMER, 1994, 35 (16) : 3576 - 3578
  • [43] THE NUCLEOTIDE-SEQUENCES OF 2 TRANSFER TRNAASN GENES FROM TOBACCO CHLOROPLASTS
    KATO, A
    SHIMADA, H
    KUSUDA, M
    SUGIURA, M
    NUCLEIC ACIDS RESEARCH, 1981, 9 (21) : 5601 - 5607
  • [44] KINETIC-STUDY OF AN ENZYMATIC LIQUID LIQUID REACTION - THE HYDROLYSIS OF TRIBUTYRIN BY CANDIDA-CYLINDRACEA LIPASE
    MARTINEZ, O
    WILHELM, AM
    RIBA, JP
    JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 1992, 53 (04) : 373 - 378
  • [45] CLONING AND NUCLEOTIDE-SEQUENCES OF THE ACCI RESTRICTION MODIFICATION GENES IN ACINETOBACTER-CALCOACETICUS
    KAWAKAMI, B
    CHRISTOPHE, H
    NAGATOMO, M
    OKA, M
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1991, 55 (06): : 1553 - 1559
  • [46] LIPASE ACTIVITY IN VESICULAR SYSTEMS - CHARACTERIZATION OF CANDIDA-CYLINDRACEA LIPASE AND ITS ACTIVITY IN POLYMERIZABLE DIALKYLAMMONIUM SURFACTANT VESICLES
    MOSMULLER, EWJ
    FRANSSEN, MCR
    ENGBERSEN, JFJ
    BIOTECHNOLOGY AND BIOENGINEERING, 1993, 42 (02) : 196 - 204
  • [47] A RULE TO PREDICT WHICH SECONDARY ALCOHOLS ARE EFFICIENTLY RESOLVED BY CHOLESTEROL ESTERASE, LIPASE FROM PSEUDOMONAS-CEPACIA, AND LIPASE FROM CANDIDA-CYLINDRACEA
    KAZLAUSKAS, RJ
    WEISSFLOCH, ANE
    CUCCIA, LA
    RAPPAPORT, A
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1990, 200 : 226 - ORGN
  • [48] IMMOBILIZATION OF CANDIDA-CYLINDRACEA LIPASE ON METHYL ACRYLATE-DIVINYL BENZENE COPOLYMER AND ITS DERIVATIVES
    XU, HX
    LI, MQ
    HE, BL
    ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (03) : 194 - 199
  • [49] ENRICHMENT OF ARACHIDONIC-ACID - SELECTIVE HYDROLYSIS OF A SINGLE-CELL OIL FROM MORTIERELLA WITH CANDIDA-CYLINDRACEA LIPASE
    SHIMADA, Y
    SUGIHARA, A
    MARUYAMA, K
    NAGAO, T
    NAKAYAMA, S
    NAKANO, H
    TOMINAGA, Y
    JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1995, 72 (11) : 1323 - 1327
  • [50] CRYSTALLIZATION AND PRELIMINARY DIFFRACTION ANALYSIS OF CHOLESTEROL ESTERASE FROM CANDIDA-CYLINDRACEA
    GHOSH, D
    ERMAN, M
    DUAX, WL
    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1991, 38 (05): : 663 - 665
12345