CLONING AND NUCLEOTIDE-SEQUENCES OF 2 LIPASE GENES FROM CANDIDA-CYLINDRACEA

被引：75
|
作者：
LONGHI, S [1 ]
FUSETTI, F [1 ]
GRANDORI, R [1 ]
LOTTI, M [1 ]
VANONI, M [1 ]
ALBERGHINA, L [1 ]
机构：
[1] UNIV MILAN, DIPARTIMENTO FISIOL & BIOCHIM GEN, SEZ BIOCHIM COMPARATA, I-20133 MILAN, ITALY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 02期
关键词：
LIPASE GENE; CLONING; GENOMIC SEQUENCE; (YEAST); (C-CYLINDRACEA);
D O I：
10.1016/0167-4781(92)90085-E
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two lipase-encoding genes (LIP1 and LIP2) have been isolated from a SacI genomic library of the yeast Candida cylindracea and their nucleotide sequences have been determined. Comparison with the sequence of a cDNA ruled out the presence of introns in the two genes. Both ORFs encode for mature proteins of 534 residues with putative signal peptides of 15 and 14 amino acids, respectively. When compared with other lipase sequences, the two C. cylindracea lipases showed homology only with the Geotrichum candidum lipase, whereas they shared a significant similarity with several esterases.
引用
收藏
页码：227 / 232
页数：6
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