PREPARATION OF [4R-H-3]NADH, [4R-H-3]NADPH AND THE CORRESPONDING 4S-ISOMERS ALL WITH SUBSTANTIAL SPECIFIC ACTIVITIES

被引：7

作者：

ICHINOSE, K ^{[1
]}

LEEPER, FJ ^{[1
]}

BATTERSBY, AR ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,CHEM LAB,LENSFIELD RD,CAMBRIDGE CB2 1EW,ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 | 1993年 / 11期

关键词：

D O I：

10.1039/p19930001213

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Methods are developed for the enzymic preparation of [4R-H-3]NADH, [4R-H-3]NADPH and the corresponding 4S-isomers, all stereospecifically labelled and of specific activities amply high enough for sensitive labelling experiments. Sodium borotritiide provides the tritium for all four cofactors.

引用

页码：1213 / 1216

页数：4

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